Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all TBC1D15 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by the antibody maps to a region between residue 1 to 50 of human TBC1 Domain Family, Member 15 using the numbering given in entry NP_073608.4.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TBC1D15 Antibody

Western Blot: TBC1D15 Antibody [NBP2-36552]

Western Blot: TBC1D15 Antibody [NBP2-36552]

Western Blot: TBC1D15 Antibody [NBP2-36552] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TBC1D15 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: TBC1D15 Antibody [NBP2-36552]

Immunoprecipitation: TBC1D15 Antibody [NBP2-36552]

Immunoprecipitation: TBC1D15 Antibody [NBP2-36552] - Detection of human TBC1D15 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TBC1D15 antibody NBP2-36552 used for IP at 6 ug per reaction. TBC1D15 was also immunoprecipitated by rabbit anti-TBC1D15 antibody NBP2-36553. For blotting immunoprecipitated TBC1D15, NBP2-36552 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for TBC1D15 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:1000 - 1:5000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TBC1D15

Alternate Names

domain family member 15, TBC1 domain family, member 15

Gene Symbol

TBC1D15

UniProt

NP_073608.4 (Human)

Additional TBC1D15 Products

Product Documents for TBC1D15 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TBC1D15 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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