Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all TCEA1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human transcription elongation factor S-II (transcription elongation factor A (SII), 1) using the numbering given in entry NP_006747.1 (GeneID 6917).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TCEA1 Antibody

Immunohistochemistry-Paraffin: TCEA1 Antibody [NBP1-26624]

Immunohistochemistry-Paraffin: TCEA1 Antibody [NBP1-26624]

Immunohistochemistry-Paraffin: TCEA1 Antibody [NBP1-26624] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- TFIIS used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunoprecipitation: TCEA1 Antibody [NBP1-26624]

Immunoprecipitation: TCEA1 Antibody [NBP1-26624]

Immunoprecipitation: TCEA1 Antibody [NBP1-26624] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit used for IP at 10 mcg/mg lysate. TFIIS was unsuccessfully immunoprecipitated by rabbit anti-TFIIS antibody NBP1-26625, which recognizes a downstream epitope.

Applications for TCEA1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Immunoprecipitation

5-10 ug/mg lysate
Application Notes
Not recommended for western analysis. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TCEA1

TFIIS is a transcription elongation factor that plays a role in RNA polymerase II transcription initiation. It also plays a role in transcript cleavage activity of arrested RNA which allows resumption of elongation from the newly cleaved 3'-prime terminus.

Alternate Names

GTF2Stranscription elongation factor A protein 1, SII, TF2STCEA, TFIISTranscription elongation factor TFIIS.o, transcription elongation factor A (SII), 1, Transcription elongation factor S-II protein 1

Entrez Gene IDs

6917 (Human)

Gene Symbol

TCEA1

UniProt

NP_006747.1 (Human)

Additional TCEA1 Products

Product Documents for TCEA1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TCEA1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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