TCF-12/HTF4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99046

Novus Biologicals, part of Bio-Techne
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NBP2-99046-100ul
NBP2-99046-50ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TCF-12/HTF4 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human TCF-12/HTF4.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for TCF-12/HTF4 Antibody

Western Blot: TCF-12/HTF4 Antibody [NBP2-99046]

Western Blot: TCF-12/HTF4 Antibody [NBP2-99046]

Western Blot: TCF-12/HTF4 Antibody [NBP2-99046] - Anti-TCF-12/HTF4 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 73 kDa. Observed band size: 85 kDa
Immunocytochemistry/ Immunofluorescence: TCF-12/HTF4 Antibody [NBP2-99046]

Immunocytochemistry/ Immunofluorescence: TCF-12/HTF4 Antibody [NBP2-99046]

Immunocytochemistry/Immunofluorescence: TCF-12/HTF4 Antibody [NBP2-99046] - Immunofluorescence staining of TCF-12/HTF4 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human TCF-12/HTF4 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to .
Immunohistochemistry-Paraffin: TCF-12/HTF4 Antibody [NBP2-99046]

Immunohistochemistry-Paraffin: TCF-12/HTF4 Antibody [NBP2-99046]

Immunohistochemistry-Paraffin: TCF-12/HTF4 Antibody [NBP2-99046] - Immunochemical staining of human TCF-12/HTF4 in human colon carcinoma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.
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Applications for TCF-12/HTF4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TCF-12/HTF4

TCF12 is encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Long Name

Transcription Factor 12

Alternate Names

BHLHB20, HEB, HTF4, TCF12

Gene Symbol

TCF12

Additional TCF-12/HTF4 Products

Product Documents for TCF-12/HTF4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TCF-12/HTF4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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