TCF-2/HNF-1 beta Antibody (4E9)

Novus Biologicals, part of Bio-Techne | Catalog # H00006928-M06

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 4E9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TCF-2/HNF-1 beta Antibodies »

Product Specifications

Immunogen

TCF2 (NP_000449, 29 a.a. ~ 118 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ALEELLPSPNFGVKLETLPLSPGSGAEPDTKPVFHTLTNGHAKGRLSGDEGSEDGDDYDTPPILKELQALNTEEAAEQRAEVDRMLSEDP

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

TCF2 - transcription factor 2, hepatic; LF-B3; variant hepatic nuclear factor (4E9)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for TCF-2/HNF-1 beta Antibody (4E9)

Western Blot: TCF-2/HNF-1 beta Antibody (4E9) [H00006928-M06]

Western Blot: TCF-2/HNF-1 beta Antibody (4E9) [H00006928-M06]

Western Blot: TCF-2/HNF-1 beta Antibody (4E9) [H00006928-M06] - Analysis of TCF2 expression in Raw 264.7 (Cat # L024V1).
Western Blot: TCF-2/HNF-1 beta Antibody (4E9) [H00006928-M06]

Western Blot: TCF-2/HNF-1 beta Antibody (4E9) [H00006928-M06]

Western Blot: TCF-2/HNF-1 beta Antibody (4E9) [H00006928-M06] - Analysis of TCF2 expression in HeLa (Cat # L013V1).
Western Blot: TCF-2/HNF-1 beta Antibody (4E9) [H00006928-M06]

Western Blot: TCF-2/HNF-1 beta Antibody (4E9) [H00006928-M06]

Western Blot: TCF-2/HNF-1 beta Antibody (4E9) [H00006928-M06] - Analysis of TCF2 expression in PC-12 (Cat # L012V1).
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Applications for TCF-2/HNF-1 beta Antibody (4E9)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TCF-2/HNF-1 beta

This gene encodes a member of the transcription factor superfamily. The encoded protein forms heterodimers with another member of this transcription factor family, HNF1A. Multiple alternatively spliced transcript variants have been described, however the biological validity all of these variants needs to be determined. [provided by RefSeq]

Long Name

Transcription Factor 2

Alternate Names

HNF-1 beta, HNF1B, HNF2, LF-B3, MODY5, TCF2, VHNF1

Entrez Gene IDs

6928 (Human)

Gene Symbol

HNF1B

UniProt

NP_000449 (Human)

Additional TCF-2/HNF-1 beta Products

Product Documents for TCF-2/HNF-1 beta Antibody (4E9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TCF-2/HNF-1 beta Antibody (4E9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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