TCF-3/E2A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-57092

Novus Biologicals, part of Bio-Techne
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NB100-57092
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all TCF-3/E2A Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KAPRARTSPDEDED corresponding to internal region according to NP_003191.1.

Epitope

KAPRARTSPDEDED

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:28402854).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for TCF-3/E2A Antibody

Immunohistochemistry-Paraffin: TCF-3/E2A Antibody [NB100-57092]

Immunohistochemistry-Paraffin: TCF-3/E2A Antibody [NB100-57092]

Immunohistochemistry-Paraffin: TCF-3/E2A Antibody [NB100-57092] - TCF3 / E2A Antibody (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
SDS-PAGE: TCF-3/E2A Antibody [NB100-57092]

SDS-PAGE: TCF-3/E2A Antibody [NB100-57092]

SDS-Page: TCF-3/E2A Antibody [NB100-57092] - Analysis of TCF3/E2A in Daudi cell lysate (35ug protein in RIPA buffer) using this antibody at 0.01ug/ml.

Applications for TCF-3/E2A Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 5 ug/ml

Immunohistochemistry-Paraffin

3 - 5 ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

0.01 - 0.03 ug/ml
Application Notes
WB: Approx. 75 kDa band observed in Daudi lysates (calculated MW of 67.6 kDa band according to NP_003191.1). IHC-P: Human kidney shows strong nuclear staining in DCT cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TCF-3/E2A

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporatic cases of maturity-onset diabetes of the young. Two transcript variants encoding the same protein have been identified for this gene.

Long Name

Transcription Factor 3/E2-alpha

Alternate Names

bHLHb21, E2A, ITF1, Kappa-E2-binding factor, Pan2, Tcfe2a, VDIR

Entrez Gene IDs

6929 (Human)

Gene Symbol

TCF3

UniProt

NP_003191.1 (Human)

Additional TCF-3/E2A Products

Product Documents for TCF-3/E2A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TCF-3/E2A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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