TCF4 Antibody (1G4)

Novus Biologicals, part of Bio-Techne | Catalog # H00006925-M03

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey, Rabbit

Cited:

Human, Rabbit

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1G4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TCF4 Antibodies »

Product Specifications

Immunogen

TCF4 (AAH31056.1, 1 a.a. ~ 365 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MHHQQRMAALGTDKELSDLLDFSAMFSPPVSSGKNGPTSLASGHFTGSNVEDRSSSGSWGNGGHPSPSRNYGDGTPYDHTTSRDLGSHDNLSPPFVNSRIQSKTERGSYSSYGRESNLQGCHQQSLLGGDMDMGNPGTLSPTKPGSQYYQYSSNNPRRRPLHSSAMEVQTKKVRKVPPGLPSSVYAPSASTADYNRDSPGYPSSKPATSTFPSSFFMQDGHHSSDPWSSSSGMNQPGYAGMLGNSSHIPQSSSYCSLHPHERLSYPSHSSADINSSLPPMSTFHRSGTNHYSTSSCTPPANGTDSIMANRGSGAAGSSQTGDALGKALASIYSPDHTNNSFSSNPSTPVGSPPSLSAGTAVWSRN

Reactivity Notes

Rat reactivity reported in customer reivew. Rabbit reactivity reported in scientific literature (PMID: 29940337).

Specificity

TCF4 (1G4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for TCF4 Antibody (1G4)

Western Blot: TCF4 Antibody (1G4) [H00006925-M03]

Western Blot: TCF4 Antibody (1G4) [H00006925-M03]

Western Blot: TCF4 Antibody (1G4) [H00006925-M03] - Analysis of TCF4 expression in K-562 (Cat # L009V1).
Immunocytochemistry/ Immunofluorescence: TCF4 Antibody (1G4) [H00006925-M03]

Immunocytochemistry/ Immunofluorescence: TCF4 Antibody (1G4) [H00006925-M03]

Immunocytochemistry/Immunofluorescence: TCF4 Antibody (1G4) [H00006925-M03] - Analysis of monoclonal antibody to TCF4 on HeLa cell. Antibody concentration 10 ug/ml
ELISA: TCF4 Antibody (1G4) [H00006925-M03]

ELISA: TCF4 Antibody (1G4) [H00006925-M03]

ELISA: TCF4 Antibody (1G4) [H00006925-M03] - Detection limit for recombinant GST tagged TCF4 is approximately 1ng/ml as a capture antibody.

Applications for TCF4 Antibody (1G4)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody reactivity against cell lysate and recombinant protein for WB. It has been useful for IF and ELISA.

Reviewed Applications

Read 1 review rated 4 using H00006925-M03 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TCF4

This gene encodes transcription factor 4, a basic helix-turn-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is expressed predominantly in pre-B-cells, although it is found in other tissues as well. Multiple alternatively spliced transcript variants that encode different proteins have been described.

Alternate Names

BHLHB19, bHLHb19E2-2, Class B basic helix-loop-helix protein 19, Immunoglobulin transcription factor 2, ITF-2, ITF2PTHS, MGC149724, SEF-2, SEF2-1, SEF2-1A, SEF2-1B, SEF2MGC149723, SL3-3 enhancer factor 2, TCF-4, transcription factor 4

Entrez Gene IDs

6925 (Human)

Gene Symbol

TCF4

OMIM

602272 (Human)

UniProt

BC031056 (Human)

Additional TCF4 Products

Product Documents for TCF4 Antibody (1G4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TCF4 Antibody (1G4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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