TCF4 Antibody (JE41-73)

Western Blot: TCF4 Antibody (JE41-73) [NBP3-33000]

Western Blot: TCF4 Antibody (JE41-73) [NBP3-33000] - Western blot analysis of TCF4 on different lysates with Rabbit anti-TCF4 antibody (NBP3-33000) at 1/1,000 dilution.

Lane 1: A549 cell lysate
Lane 2: NIH/3T3 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Caco-2 cell lysate
Lane 5: SW620 cell lysate
Lane 6: HeLa cell lysate
Lane 7: 293T cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 71 kDa
Observed band size: 55 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33000) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.