TCF4 Antibody (JE41-73)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33000
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE41-73
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within Human TCF4 aa 321-570 / 667. (Uniprot: P15884)
Localization
Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TCF4 Antibody (JE41-73)
Western Blot: TCF4 Antibody (JE41-73) [NBP3-33000]
Western Blot: TCF4 Antibody (JE41-73) [NBP3-33000] - Western blot analysis of TCF4 on different lysates with Rabbit anti-TCF4 antibody (NBP3-33000) at 1/1,000 dilution.Lane 1: A549 cell lysate
Lane 2: NIH/3T3 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Caco-2 cell lysate
Lane 5: SW620 cell lysate
Lane 6: HeLa cell lysate
Lane 7: 293T cell lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 71 kDa
Observed band size: 55 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33000) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.
Applications for TCF4 Antibody (JE41-73)
Application
Recommended Usage
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TCF4
Alternate Names
BHLHB19, bHLHb19E2-2, Class B basic helix-loop-helix protein 19, Immunoglobulin transcription factor 2, ITF-2, ITF2PTHS, MGC149724, SEF-2, SEF2-1, SEF2-1A, SEF2-1B, SEF2MGC149723, SL3-3 enhancer factor 2, TCF-4, transcription factor 4
Gene Symbol
TCF4
Additional TCF4 Products
Product Documents for TCF4 Antibody (JE41-73)
Product Specific Notices for TCF4 Antibody (JE41-73)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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