Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # TCF4/1705

Format

Azide and BSA Free

Concentration

1 mg/ml

View all TCF4 Antibodies »

Product Specifications

Immunogen

Recombinant human TCF4 protein fragment (around aa 365-671) (exact sequence is proprietary) (Uniprot: P15884 )

Localization

Nucleus, Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07336).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for TCF4 Antibody (TCF4/1705) - Azide and BSA Free

Western Blot: TCF4 Antibody (TCF4/1705)Azide and BSA Free [NBP3-08236]

Western Blot: TCF4 Antibody (TCF4/1705)Azide and BSA Free [NBP3-08236]

Western Blot: TCF4 Antibody (TCF4/1705) - Azide and BSA Free [NBP3-08236] - Western Blot (1) HeLa and (2) HepG2 cell lysate using TCF4 Mouse Monoclonal Antibody (TCF4/1705).
Immunohistochemistry-Paraffin: TCF4 Antibody (TCF4/1705) - Azide and BSA Free [NBP3-08236]

Immunohistochemistry-Paraffin: TCF4 Antibody (TCF4/1705) - Azide and BSA Free [NBP3-08236]

Immunohistochemistry-Paraffin: TCF4 Antibody (TCF4/1705) - Azide and BSA Free [NBP3-08236] - Formalin-fixed, paraffin-embedded human bladder stained with TCF4 Antibody (TCF4/1705) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Protein Array: TCF4 Antibody (TCF4/1705) - Azide and BSA Free [NBP3-08236]

Protein Array: TCF4 Antibody (TCF4/1705) - Azide and BSA Free [NBP3-08236]

Protein Array: TCF4 Antibody (TCF4/1705) - Azide and BSA Free [NBP3-08236] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TCF4 Mouse Monoclonal Antibody (TCF4/1705).

Applications for TCF4 Antibody (TCF4/1705) - Azide and BSA Free

Application
Recommended Usage

ELISA

2-4 ug/ml

Western Blot

1-2 ug/ml
Application Notes
ELISA: Use antibody at 2-4ug/ml. For coating, order antibody without BSA

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: TCF4

TCF4 encodes transcription factor 4, a basic helix-turn-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is expressed predominantly in pre-B-cells, although it is found in other tissues as well. Multiple alternatively spliced transcript variants that encode different proteins have been described.

Alternate Names

BHLHB19, bHLHb19E2-2, Class B basic helix-loop-helix protein 19, Immunoglobulin transcription factor 2, ITF-2, ITF2PTHS, MGC149724, SEF-2, SEF2-1, SEF2-1A, SEF2-1B, SEF2MGC149723, SL3-3 enhancer factor 2, TCF-4, transcription factor 4

Gene Symbol

TCF4

Additional TCF4 Products

Product Documents for TCF4 Antibody (TCF4/1705) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TCF4 Antibody (TCF4/1705) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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