Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2c Kappa Clone # TCF4/2252

Concentration

0.2 mg/ml

View all TCF4 Antibodies »

Product Specifications

Immunogen

Recombinant human TCF4 protein fragment (around aa 365-671) (exact sequence is proprietary) (Uniprot: P15884 )

Localization

Nucleus, Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2c Kappa

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14192)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for TCF4 Antibody (TCF4/2252)

Immunohistochemistry-Paraffin: TCF4 Antibody (TCF4/2252) [NBP3-13789]

Immunohistochemistry-Paraffin: TCF4 Antibody (TCF4/2252) [NBP3-13789]

Immunohistochemistry-Paraffin: TCF4 Antibody (TCF4/2252) [NBP3-13789] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with TCF4 antibody (TCF4/2252) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Immunohistochemistry-Paraffin: TCF4 Antibody (TCF4/2252) [NBP3-13789]

Immunohistochemistry-Paraffin: TCF4 Antibody (TCF4/2252) [NBP3-13789]

Immunohistochemistry-Paraffin: TCF4 Antibody (TCF4/2252) [NBP3-13789] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with TCF4 antibody (TCF4/2252) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.

Applications for TCF4 Antibody (TCF4/2252)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA

Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: TCF4

TCF4 encodes transcription factor 4, a basic helix-turn-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is expressed predominantly in pre-B-cells, although it is found in other tissues as well. Multiple alternatively spliced transcript variants that encode different proteins have been described.

Alternate Names

BHLHB19, bHLHb19E2-2, Class B basic helix-loop-helix protein 19, Immunoglobulin transcription factor 2, ITF-2, ITF2PTHS, MGC149724, SEF-2, SEF2-1, SEF2-1A, SEF2-1B, SEF2MGC149723, SL3-3 enhancer factor 2, TCF-4, transcription factor 4

Gene Symbol

TCF4

UniProt

P15884 (Human)

Additional TCF4 Products

Product Documents for TCF4 Antibody (TCF4/2252)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TCF4 Antibody (TCF4/2252)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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