Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1-21

Format

BSA Free

Concentration

0.5 mg/ml

View all TCL1A Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was TCL1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for TCL1A Antibody (1-21) - BSA Free

Immunohistochemistry-Paraffin: TCL1A Antibody (1-21) - BSA Free [NBP1-43253]

Immunohistochemistry-Paraffin: TCL1A Antibody (1-21) - BSA Free [NBP1-43253]

Immunohistochemistry-Paraffin: TCL1A Antibody (1-21) [NBP1-43253] - Human spleen tissue stained with TCL1A Antibody (1-21). IHC-P image submitted by a verified customer review.
Flow Cytometry: TCL1A Antibody (1-21) - BSA Free [NBP1-43253]

Flow Cytometry: TCL1A Antibody (1-21) - BSA Free [NBP1-43253]

Flow Cytometry: TCL1 Antibody (1-21) [NBP1-43253] - Staining of Daudi cells with 0.25 ug of Mouse IgG2b kappa Isotype Control Purified (open histogram) or 0.25 ug of Anti-Human TCL1 Purified (filled histogram) followed by Anti-Mouse IgG Biotin and Streptavidin PE . Total viable cells were used for analysis

Applications for TCL1A Antibody (1-21) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
This 1-21 antibody has been tested by western blot analysis of Daudi cell lysates and intracellular staining followed by flow cytometric analysis of Daudi cells. For flow cytometry, this antibody can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Reviewed Applications

Read 1 review rated 5 using NBP1-43253 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TCL1A

The 1-21 antibody reacts with human T cell leukemia/lymphoma 1 (TCL1), a 14 kDa proto-oncogene product with a suggested role in intracellular regulation of T cell signalling. TCL1 was identified as the oncogene located at the 14q32.1 chromosome breakpoint region in T-cell prolymphocytic leukemia (T-PLL). In T-PLL, TCL1 is overexpressed as a result of an inversion or a reciprocal translocation, by juxtaposition to the T-cell receptor promoter/enhancer elements. TCL1 binds to the pleckstrin homology domain of Akt (protein kinase B) family proteins, which facilitates Akt dimerization and activity. By increasing Akt activity TCL1 may enhance the serine/threonine phosphorylation of major Akt signaling substrates, such as Ikk complex, mTOR, BAD, p70S6 kinase, FOXO transcription factors, and GSK3beta. These substrates regulate cellular differentiation, growth, survival, and metabolism. Besides its tumorigenic role in T-PLL, TCL1 is normally expressed in the CD3-CD4-CD8- subset of thymic precursors in the T cell lineage, the plasmacytoid subset of dendritic cells, stimulated (not resting) mature T cells, and B cells up to the germinal center stage of maturation. TCL1 is inappropriately expressed by chromosome rearrangements that lead to pre-malignant clonal T cell expansions and mature T cell tumors. TCL1 shows a regulated expression pattern in chronic lymphocytic leukemia (CLL).

Long Name

T Cell Leukemia Protein 1A

Alternate Names

TCL1-PEN

Gene Symbol

TCL1A

Additional TCL1A Products

Product Documents for TCL1A Antibody (1-21) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TCL1A Antibody (1-21) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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