TCP1 alpha Antibody (3G9G8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16290

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16290-100ul
NBP3-16290-20ul
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3G9G8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TCP1 alpha Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TCP1 alpha (P17987). MEGPLSVFGDRSTGETIRSQNVMAAASIANIVKSSLGPVGLDKMLVDDIGDVTITNDGATILKLLEVEHPAAKVLCELADLQDKEVGDGTTSVVIIAAEL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TCP1 alpha Antibody (3G9G8)

Western Blot: TCP1 alpha Antibody (3G9G8) [NBP3-16290]

Western Blot: TCP1 alpha Antibody (3G9G8) [NBP3-16290]

Western Blot: TCP1 alpha Antibody (3G9G8) [NBP3-16290] - Western blot analysis of extracts of Jurkat cells, using TCP1 alpha antibody (NBP3-16290) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry-Paraffin: TCP1 alpha Antibody (3G9G8) [NBP3-16290]

Immunohistochemistry-Paraffin: TCP1 alpha Antibody (3G9G8) [NBP3-16290]

Immunohistochemistry-Paraffin: TCP1 alpha Antibody (3G9G8) [NBP3-16290] - Immunohistochemistry of paraffin-embedded human colon using TCP1 alpha Rabbit mAb (NBP3-16290) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for TCP1 alpha Antibody (3G9G8)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TCP1 alpha

TCP1 alpha encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized.

Alternate Names

CCT1T-complex protein 1 subunit alpha, CCTA, CCT-alpha, D6S230E, tailless complex polypeptide 1, t-complex 1, T-complex protein 1, alpha subunit, TCP-1-alpha

Gene Symbol

TCP1

Additional TCP1 alpha Products

Product Documents for TCP1 alpha Antibody (3G9G8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TCP1 alpha Antibody (3G9G8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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