TCP1 alpha Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88149

Novus Biologicals, part of Bio-Techne
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NBP1-88149
NBP1-88149-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TCP1 alpha Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VRYINENLIVNTDELGRDCLINAAKTSMSSKIIGINGDFFANMVVDAVLAIKYTDIRGQPRYPVNSVNILKAHGRSQMESMLISGY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TCP1 alpha Antibody

Western Blot: TCP1 alpha Antibody [NBP1-88149]

Western Blot: TCP1 alpha Antibody [NBP1-88149]

Western Blot: TCP1 alpha Antibody [NBP1-88149] - Analysis using Anti-TCP1 antibody NBP1-88149 (A) shows similar pattern to independent antibody NBP1-88148 (B).
Immunohistochemistry-Paraffin: TCP1 alpha Antibody [NBP1-88149]

Immunohistochemistry-Paraffin: TCP1 alpha Antibody [NBP1-88149]

Immunohistochemistry-Paraffin: TCP1 alpha Antibody [NBP1-88149] - Staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferus ducts.
Western Blot: TCP1 alpha Antibody [NBP1-88149]

Western Blot: TCP1 alpha Antibody [NBP1-88149]

Western Blot: TCP1 alpha Antibody [NBP1-88149] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Applications for TCP1 alpha Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TCP1 alpha

TCP1 alpha encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized.

Alternate Names

CCT1T-complex protein 1 subunit alpha, CCTA, CCT-alpha, D6S230E, tailless complex polypeptide 1, t-complex 1, T-complex protein 1, alpha subunit, TCP-1-alpha

Gene Symbol

TCP1

Additional TCP1 alpha Products

Product Documents for TCP1 alpha Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TCP1 alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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