TCTN1 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-94088

Novus Biologicals, part of Bio-Techne
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NBP2-94088-0.02ml
NBP2-94088-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TCTN1 Antibodies »

Product Summary for TCTN1 Antibody - Azide and BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 130-310 of human TCTN1 (NP_001076006.1). NFTANPPQRVFELVDQINPSIFCIHITNYKPALSFINPEVPDENNFDTLMKTSDGFTLNAESYVSFTTKLDIPTAAKYEYGVPLQTSDSFLRFPSSLTSSLCTDNNPAAFLVNQAVKCTRKINLEQCEEIEALSMAFYSSPEILRVPDSRKKVPITVQSIVIQSLNKTLTRREDTDVLQPT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TCTN1 Antibody - Azide and BSA Free

Western Blot: TCTN1 AntibodyAzide and BSA Free [NBP2-94088]

Western Blot: TCTN1 AntibodyAzide and BSA Free [NBP2-94088]

Western Blot: TCTN1 Antibody [NBP2-94088] - Analysis of extracts of various cell lines, using TCTN1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.
Immunocytochemistry/ Immunofluorescence: TCTN1 Antibody - Azide and BSA Free [NBP2-94088]

Immunocytochemistry/ Immunofluorescence: TCTN1 Antibody - Azide and BSA Free [NBP2-94088]

Immunocytochemistry/Immunofluorescence: TCTN1 Antibody [NBP2-94088] - Immunofluorescence analysis of U2OS cells using TCTN1 Rabbit pAb (NBP2-94088) at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for TCTN1 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TCTN1

TCTN1 encodes a member of the tectonic family of secreted and transmembrane proteins. The orthologous gene in mouse is required for formation of most ventral cell types. It functions downstream of smoothened and rab23 to modulate hedgehog signal transduction. Multiple transcript variants encoding different isoforms have been found for this gene. Transcript Variant: This variant (3) uses an alternate exon combination in the central coding region and an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 3).

Long Name

Tectonic Family Member 1

Alternate Names

JBTS13, TECT1, Tectonic-1

Gene Symbol

TCTN1

Additional TCTN1 Products

Product Documents for TCTN1 Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TCTN1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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