TDAG8/GPR65 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21356

Novus Biologicals, part of Bio-Techne
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NBP3-21356-100ul
NBP3-21356-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TDAG8/GPR65 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LLIRCILEHAVNFEDHSNSGKRTYTMYRI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TDAG8/GPR65 Antibody

Immunohistochemistry-Paraffin: TDAG8/GPR65 Antibody [NBP3-21356] -

Immunohistochemistry-Paraffin: TDAG8/GPR65 Antibody [NBP3-21356] -

Staining of human spleen shows high expression.
Western Blot: TDAG8/GPR65 Antibody [NBP3-21356] -

Western Blot: TDAG8/GPR65 Antibody [NBP3-21356] -

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil
Immunohistochemistry-Paraffin: TDAG8/GPR65 Antibody [NBP3-21356] -

Immunohistochemistry-Paraffin: TDAG8/GPR65 Antibody [NBP3-21356] -

Staining of human skeletal muscle shows low expression as expected.
View 1 more images

Applications for TDAG8/GPR65 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TDAG8/GPR65

GPR65 (TDAG8), a Lysophospholipid/Lysosphingolipid Receptor, is a molecular target for psychosine, a toxic lipid formed by the breakdown of galactosylceramide (cerebroside). A build-up of psychosine, caused by the lack of galactosylceramidase, results in the death of the myelin-synthesizing cells, oligodendrocytes, which is associated with Krabbe disease. GPR65 has been reported in human in peripheral blood leukocytes, spleen, lymph node, and thymus. ESTs have been isolated from human B-cell/lung/testis, brain/lung/testis, pancreas/spleen, lung/spleen, leukocyte, embryo, kidney, liver/spleen, thyroid, and vessel libraries.

Long Name

G Protein-coupled Receptor 65

Alternate Names

GPR65, Psychosine Receptor

Gene Symbol

GPR65

Additional TDAG8/GPR65 Products

Product Documents for TDAG8/GPR65 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TDAG8/GPR65 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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