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TDG Antibody (311)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43802

Novus Biologicals, part of Bio-Techne
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NBP2-43802

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (99%), Chicken (95%), Rat (99%), Xenopus (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 311

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for TDG Antibody (311)

Immunogen

Recombinant protein encompassing a sequence within the center region of human TDG. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TDG Antibody (311)

Western Blot: TDG Antibody (311) [NBP2-43802]

Western Blot: TDG Antibody (311) [NBP2-43802]

Western Blot: TDG Antibody (311) [NBP2-43802] - Analysis of Non-transfected (-) and TDG -transfected (+, ) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TDG antibody diluted by 1:2500.
Immunohistochemistry-Paraffin: TDG Antibody (311) [NBP2-43802]

Immunohistochemistry-Paraffin: TDG Antibody (311) [NBP2-43802]

Immunohistochemistry-Paraffin: TDG Antibody (311) [NBP2-43802] - TDG antibody detects TDG protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain. TDG stained by TDG antibody diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
TDG Antibody (311)

Western Blot: TDG Antibody (311) [NBP2-43802] -

Western Blot: TDG Antibody (311) [NBP2-43802] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TDG antibody [GT311] (NBP2-43802) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for TDG Antibody (311)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TDG

Thymine DNA glycosylase is encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq]

Long Name

Thymine DNA Glycosylase

Alternate Names

EC 3.2.2.29, thymine-DNA glycosylaseG/T mismatch-specific thymine DNA glycosylase

Gene Symbol

TDG

Additional TDG Products

Product Documents for TDG Antibody (311)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TDG Antibody (311)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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