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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Chicken (100%), Rat (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 601 and 651 of human tudor domain containing 3 using the numbering given in entry NP_110421.1 (GeneID 81550).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TDRD3 Antibody

Western Blot: TDRD3 Antibody [NBP1-46821]

Western Blot: TDRD3 Antibody [NBP1-46821]

Western Blot: TDRD3 Antibody [NBP1-46821] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TDRD3 antibody NBP1-46821 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. TDRD3 was also immunoprecipitated by rabbit anti-TDRD3 antibody NBP1-46820, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Immunohistochemistry-Paraffin: TDRD3 Antibody [NBP1-46821]

Immunohistochemistry-Paraffin: TDRD3 Antibody [NBP1-46821]

Immunohistochemistry-Paraffin: TDRD3 Antibody [NBP1-46821] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti- TDRD3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Applications for TDRD3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500- 1:2000

Immunohistochemistry-Paraffin

1:500- 1:2000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TDRD3

TDRD3 may play a role in the assembly and/or disassembly of mRNA stress granules and in the regulation oftranslation of target mRNAs. Binds Arg/Gly-rich motifs in dimethylarginine-containing proteins

Alternate Names

FLJ21007, tudor domain containing 3, tudor domain-containing protein 3

Gene Symbol

TDRD3

Additional TDRD3 Products

Product Documents for TDRD3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TDRD3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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