TdT Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-58254

Novus Biologicals, part of Bio-Techne
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NBP1-58254
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all TdT Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to DNTT(deoxynucleotidyltransferase, terminal) The peptide sequence was selected from the N terminal of DNTT. Peptide sequence FQDLVVFILEKKMGTTRRAFLMELARRKGFRVENELSDSVTHIVAENNSG. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for TdT Antibody

Western Blot: TdT Antibody [NBP1-58254]

Western Blot: TdT Antibody [NBP1-58254]

Western Blot: TdT Antibody [NBP1-58254] - Jurkat cell lysate, concentration 0.2-1 ug/ml.

Applications for TdT Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TdT

DNTT is a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, DNTT is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.

Long Name

Terminal Deoxynucleotidyl Transferase

Alternate Names

DNTT

Gene Symbol

DNTT

UniProt

P04053 (Human)

Additional TdT Products

Product Documents for TdT Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TdT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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