TEAD1 Antibody (2E7N10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16593
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2E7N10
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for TEAD1 Antibody (2E7N10)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TEAD1 (P28347). MEPSSWSGSESPAENMERMSDSADKPIDNDAEGVWSPDIEQSFQEALAIYPPCGRRKIILSDEGKMYGRNELIARYIKLRTGKTRTRKQVSSHIQVLARR
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TEAD1 Antibody (2E7N10)
![Western Blot: TEF1 Antibody (2E7N10) [NBP3-16593]](https://resources.bio-techne.com/images/products/TEF1-Antibody-2E7N10-Western-Blot-NBP3-16593-img0001.jpg "Western Blot: TEF1 Antibody (2E7N10) [NBP3-16593]")
Western Blot: TEF1 Antibody (2E7N10) [NBP3-16593]
Western Blot: TEF1 Antibody (2E7N10) [NBP3-16593] - Western blot analysis of extracts of various cell lines, using TEF1 Rabbit mAb (NBP3-16593) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.![Immunoprecipitation: TEF1 Antibody (2E7N10) [NBP3-16593]](https://resources.bio-techne.com/images/products/TEF1-Antibody-2E7N10-Immunoprecipitation-NBP3-16593-img0002.jpg "Immunoprecipitation: TEF1 Antibody (2E7N10) [NBP3-16593]")
Immunoprecipitation: TEF1 Antibody (2E7N10) [NBP3-16593]
Immunoprecipitation: TEF1 Antibody (2E7N10) [NBP3-16593] - Immunoprecipitation analysis of 600ug extracts of Mouse skeletal muscle cells using 3ug TEF1 antibody (NBP3-16593). Western blot was performed from the immunoprecipitate using TEF1 antibody (NBP3-16593) at a dilition of 1:1000.
Chromatin Immunoprecipitation: TEF1 Antibody (2E7N10) [NBP3-16593] -
Chromatin Immunoprecipitation: TEF1 Antibody (2E7N10) [NBP3-16593] - Analysis of extracts of HepG2 cells, using TEAD1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.Applications for TEAD1 Antibody (2E7N10)
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
5ug antibody for 10ug-15ug of Chromatin
Immunoprecipitation
0.5ug-4ug antibody for 400ug-600ug extracts of whole cells
Western Blot
1:500 - 1:2000
Application Notes
CUT&Tag 10 cells /1 ug
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: TEAD1
Long Name
Transcriptional enhancer factor TEF-1
Alternate Names
AA, NTEF-1, Protein GT-IIC, REF1, TCF13, TCF-13, TEA domain family member 1 (SV40 transcriptional enhancer factor), TEAD1, TEAD-1, TEF-1, transcription factor 13, transcriptional enhancer factor 1, transcriptional enhancer factor TEF-1
Gene Symbol
TEAD1
Additional TEAD1 Products
Product Documents for TEAD1 Antibody (2E7N10)
Product Specific Notices for TEAD1 Antibody (2E7N10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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