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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (94%). Backed by our 100% Guarantee.

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Antibody was raised against a 18 amino acid synthetic peptide near the center of human TEM4. The immunogen is located within amino acids 910 - 960 of TEM4. Amino Acid Squence: KKRPARSSHQELRRDEG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

200 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TEM4 Antibody - BSA Free

Western Blot: TEM4 AntibodyBSA Free [NBP1-77314]

Western Blot: TEM4 AntibodyBSA Free [NBP1-77314]

Western Blot: TEM4 Antibody [NBP1-77314] - Analysis of TEM4 in Jurkat cell lysate with TEM4 antibody at (A) 0.5 and (B) 1 ug/mL.
Immunohistochemistry-Paraffin: TEM4 Antibody - BSA Free [NBP1-77314]

Immunohistochemistry-Paraffin: TEM4 Antibody - BSA Free [NBP1-77314]

Immunohistochemistry-Paraffin: TEM4 Antibody [NBP1-77314] - Human spleen tissue with TEM4 antibody at 2.5 ug/ml.

Applications for TEM4 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

0.5-1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TEM4

Rho GTPases, which are activated by specific guanine-nucleotide exchange factors (GEFs), play pivotal roles in several cellular functions. TEM4, encoding a protein containing 1510 amino acids, contains a RhoGEF-specific Dbl homology (DH) domain but lacks their typical pleckstrin homology domain. TEM4 is a Rho-specific GEF with novel structural and regulatory properties and predominant expression in the heart. It couples tyrosine kinase signals with the activation of the rho/rac GTPases, thus leading to cell differentiation and/or proliferation. Elevated levels of TEM4, TEM5, TEM6, TEM7 and TEM7R were also raised in breast cancer tissues. TEM4 could also prove to be useful targets therapeutically.

Alternate Names

164 kDa Rho-specific guanine-nucleotide exchange factor, FLJ90019, KIAA0337P164RHOGEF, p164RhoGEF, p164-RhoGEFRho-specific guanine-nucleotide exchange factor 164 kDa, Rho guanine nucleotide exchange factor (GEF) 17, TEM4rho guanine nucleotide exchange factor 17, Tumor endothelial marker 4

Gene Symbol

ARHGEF17

UniProt

Additional TEM4 Products

Product Documents for TEM4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TEM4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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