TET3 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59669
![Immunohistochemistry-Paraffin: TET3 Antibody - BSA Free [NBP2-59669]](https://resources.bio-techne.com/images/products/TET3-Antibody-Immunohistochemistry-Paraffin-NBP2-59669-img0001.jpg "Immunohistochemistry-Paraffin: TET3 Antibody - BSA Free [NBP2-59669]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Partial recombinant protein made to an N-terminal portion of human TET3 (amino acids 1-216) [UniProt O43151]
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TET3 Antibody - BSA Free
Immunohistochemistry-Paraffin: TET3 Antibody - BSA Free [NBP2-59669]
Immunohistochemistry-Paraffin: TET3 Antibody [NBP2-59669] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse colon using TET3 antibody at 1:150 dilution. The signal was developed using HRP-DAB method and the nuclei were counterstained using hematoxylin. This antibody detected TET3 protein in the cytoplasm and the nuclei of the columnar epithelial cells and the goblet cells.![Western Blot: TET3 AntibodyBSA Free [NBP2-59669]](https://resources.bio-techne.com/images/products/TET3-Antibody-Western-Blot-NBP2-59669-img0003.jpg "Western Blot: TET3 AntibodyBSA Free [NBP2-59669]")
Western Blot: TET3 AntibodyBSA Free [NBP2-59669]
Western Blot: TET3 Antibody [NBP2-59669] - Partial recombinant TET3 protein was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-TET3 in block buffer detected with an anti-rabbit HRP secondary antibody using chemiluminescence.![Immunocytochemistry/ Immunofluorescence: TET3 Antibody - BSA Free [NBP2-59669]](https://resources.bio-techne.com/images/products/TET3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-59669-img0002.jpg "Immunocytochemistry/ Immunofluorescence: TET3 Antibody - BSA Free [NBP2-59669]")
Immunocytochemistry/ Immunofluorescence: TET3 Antibody - BSA Free [NBP2-59669]
Immunocytochemistry/Immunofluorescence: TET3 Antibody [NBP2-59669] - NIH/3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-TET3 (NBP2-59669) at 5ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.Applications for TET3 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
5 ug/ml
Immunohistochemistry
1:100 - 1:500
Immunohistochemistry-Paraffin
1:100 - 1:500
Western Blot
0.5 - 2.0 ug/ml
Application Notes
Tested in Western blot on recombinant protein.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TET3
Alternate Names
EC 1.14.11.n2, hCG_40738, KIAA0401, methylcytosine dioxygenase TET3, MGC22014, probable methylcytosine dioxygenase TET3, tet oncogene family member 3
Gene Symbol
TET3
Additional TET3 Products
Product Documents for TET3 Antibody - BSA Free
Product Specific Notices for TET3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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