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TFAP4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46202

Novus Biologicals, part of Bio-Techne
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NBP1-46202

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Summary for TFAP4 Antibody

Immunogen

The immunogen for this product maps to a region between residue 288 and 338 of human Activating Enhancer-Binding Protein 4 using the numbering given in entry NP_003214.1 (GeneID 7023).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TFAP4 Antibody

Western Blot: TFAP4 Antibody [NBP1-46202]

Western Blot: TFAP4 Antibody [NBP1-46202]

Western Blot: TFAP4 Antibody [NBP1-46202] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-AP4 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. AP4 was also immunoprecipitated by rabbit anti-AP4 antibody NBP1-46201, which recognizes an upstream epitope.

Applications for TFAP4 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TFAP4

Transcription factors of the basic helix-loop-helix-zipper (bHLH-ZIP) family contain a basic domain, which is used for DNA binding, and HLH and ZIP domains, which are used for oligomerization. Transcription factor AP4 activates both viral and cellular genes by binding to the symmetrical DNA sequence CAGCTG (Mermod et al., 1988 [PubMed 2833704]; Hu et al., 1990 [PubMed 2123466]).[supplied by OMIM]

Alternate Names

Activating enhancer-binding protein 4, AP-4, BHLHC41, bHLHc41Class C basic helix-loop-helix protein 41, transcription factor AP-4, transcription factor AP-4 (activating enhancer binding protein 4), transcription factor AP-4 (activating enhancer-binding protein 4)

Gene Symbol

TFAP4

Additional TFAP4 Products

Product Documents for TFAP4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TFAP4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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