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TFB1M Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13429

Novus Biologicals, part of Bio-Techne
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NBP2-13429
NBP2-13429-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: QRSRLSVMAQYLCNVRHIFTIPGQAFVPKPEVDVGVVHFTPLIQPKIEQPFKLVEKVVQNVFQFRRKYCHRGLRMLFPEAQRLESTGRLLELADIDPTLRPRQLSISHFKSLCDVYRKMCDEDPQL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TFB1M Antibody

Western Blot: TFB1M Antibody [NBP2-13429]

Western Blot: TFB1M Antibody [NBP2-13429]

Western Blot: TFB1M Antibody [NBP2-13429] - Analysis in control (vector only transfected HEK293T lysate) and TFB1M over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: TFB1M Antibody [NBP2-13429]

Immunohistochemistry-Paraffin: TFB1M Antibody [NBP2-13429]

Immunohistochemistry-Paraffin: TFB1M Antibody [NBP2-13429] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.

Applications for TFB1M Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TFB1M

The transcription of genes from mitochondrial DNA requires a mitochondrial RNA polymerase (see POLRMT, MIM 601778) and a DNA-binding transcription factor (see TFAM, MIM 600438). Transcription factor B1 (TFB1M) is a part of this transcription complex

Alternate Names

CGI75, CGI-75, dimethyladenosine transferase 1, mitochondrial, EC 2.1.1, EC 2.1.1.-, h-mtTFB, h-mtTFB1, homolog of yeast mitochondrial transcription factor B, hTFB1M, Mitochondrial 12S rRNA dimethylase 1, mitochondrial dimethyladenosine transferase 1, Mitochondrial transcription factor B1, mtTFB, mtTFB1, S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 1, transcription factor B1, mitochondrial

Gene Symbol

TFB1M

Additional TFB1M Products

Product Documents for TFB1M Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TFB1M Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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