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TFB2M Antibody (2E10)

Novus Biologicals, part of Bio-Techne | Catalog # H00064216-M01

Novus Biologicals, part of Bio-Techne
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H00064216-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2E10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

TFB2M (NP_071761, 297 a.a. ~ 396 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YLIQMIPRQNLFTKNLTPMNYNIFFHLLKHCFGRRSATVIDHLRSLTPLDARDILMQIGKQEDEKVVNMHPQDFKTLFETIERSKDCAYKWLYDETLEDR

Specificity

TFB2M - transcription factor B2, mitochondrial (2E10)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for TFB2M Antibody (2E10)

Western Blot: TFB2M Antibody (2E10) [H00064216-M01]

Western Blot: TFB2M Antibody (2E10) [H00064216-M01]

Western Blot: TFB2M Antibody (2E10) [H00064216-M01] - TFB2M monoclonal antibody (M01), clone 2E10. Analysis of TFB2M expression in HeLa.
Western Blot: TFB2M Antibody (2E10) [H00064216-M01]

Western Blot: TFB2M Antibody (2E10) [H00064216-M01]

Western Blot: TFB2M Antibody (2E10) [H00064216-M01] - TFB2M monoclonal antibody (M01), clone 2E10. Analysis of TFB2M expression in human kidney.
Sandwich ELISA: TFB2M Antibody (2E10) [H00064216-M01] - Detection limit for recombinant GST tagged TFB2M is 0.03 ng/ml as a capture antibody.

Applications for TFB2M Antibody (2E10)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TFB2M

S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA atthe conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction withPOLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, itactivates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity

Alternate Names

dimethyladenosine transferase 2, mitochondrial, EC 2.1.1.-, FLJ22661, FLJ23182, HCV NS5A-transactivated protein 5, Hepatitis C virus NS5A-transactivated protein 5, Hkp1, h-mtTFB, h-mtTFB2, hTFB2M, Mitochondrial 12S rRNA dimethylase 2, Mitochondrial transcription factor B2, mtTFB2, NS5ATP5, S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 2, transcription factor B2, mitochondrial

Entrez Gene IDs

64216 (Human)

Gene Symbol

TFB2M

Additional TFB2M Products

Product Documents for TFB2M Antibody (2E10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TFB2M Antibody (2E10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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