Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # PS2GE2

Format

BSA Free

Concentration

1 mg/ml

View all TFF1 Antibodies »

Product Summary for TFF1/pS2 Antibody (PS2GE2) - BSA Free

Immunogen

A synthetic peptide of 28 amino acid residues from the C-terminus of human TFF1/pS2 protein

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

6.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TFF1/pS2 Antibody (PS2GE2) - BSA Free

Immunohistochemistry: TFF1/pS2 Antibody (PS2GE2) - BSA Free [NBP2-50275]

Immunohistochemistry: TFF1/pS2 Antibody (PS2GE2) - BSA Free [NBP2-50275]

Immunohistochemistry: TFF1/pS2 Antibody (PS2GE2) [NBP2-50275] - TFF1/pS2 Antibody (PS2GE2)
Immunohistochemistry: TFF1/pS2 Antibody (PS2GE2) - BSA Free [NBP2-50275]

Immunohistochemistry: TFF1/pS2 Antibody (PS2GE2) - BSA Free [NBP2-50275]

Immunohistochemistry: TFF1/pS2 Antibody (PS2GE2) [NBP2-50275] - TFF1/pS2 Antibody (PS2GE2)

Applications for TFF1/pS2 Antibody (PS2GE2) - BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 - 1.0 ug/10^6 cells

Immunocytochemistry/ Immunofluorescence

0.5 - 1 ug/ml

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

0.5 - 1 ug/ml

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. This antibody is Cytof ready. Positive control(s): Normal stomach, breast or ovarian carcinoma.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TFF1

Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21.

Long Name

Trefoil Factor 1

Alternate Names

BCEI, D21S21, HPS2, PNR-2, PS2

Gene Symbol

TFF1

Additional TFF1 Products

Product Documents for TFF1/pS2 Antibody (PS2GE2) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TFF1/pS2 Antibody (PS2GE2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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