TFIIE-alpha Antibody (JE66-41)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33006
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE66-41
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human TFIIE-alpha aa 390-439 / 439. (Uniprot: P29083)
Localization
Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
49.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TFIIE-alpha Antibody (JE66-41)
Western Blot: TFIIE-alpha Antibody (JE66-41) [NBP3-33006]
Western Blot: TFIIE-alpha Antibody (JE66-41) [NBP3-33006] - Western blot analysis of TFIIE-alpha on different lysates with Rabbit anti-TFIIE-alpha antibody (NBP3-33006) at 1/1,000 dilution.Lane 1: K-562 cell lysate (15 ug/Lane)
Lane 2: Jurkat cell lysate (15 ug/Lane)
Lane 3: Daudi cell lysate (15 ug/Lane)
Lane 4: HeLa cell lysate (15 ug/Lane)
Lane 5: A431 cell lysate (15 ug/Lane)
Lane 6: A549 cell lysate (15 ug/Lane)
Lane 7: HepG2 cell lysate (15 ug/Lane)
Lane 8: HEK-293 cell lysate (15 ug/Lane)
Lane 9: NIH/3T3 cell lysate (15 ug/Lane)
Lane 10: MCF7 cell lysate (15 ug/Lane)
Lane 11: Mouse testis tissue lysate (30 ug/Lane)
Lane 12: Rat testis tissue lysate (30 ug/Lane)
Predicted band size: 49.5 kDa
Observed band size: 60 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33006) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Applications for TFIIE-alpha Antibody (JE66-41)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TFIIE-alpha
Alternate Names
FE, General transcription factor IIE 56 kDa subunit, general transcription factor IIE subunit 1, general transcription factor IIE, polypeptide 1 (alpha subunit, 56kD), general transcription factor IIE, polypeptide 1, alpha 56kDa, TF2E1, TFIIE-A, TFIIE-alpha, Transcription initiation factor IIE subunit alpha
Gene Symbol
GTF2E1
Additional TFIIE-alpha Products
Product Documents for TFIIE-alpha Antibody (JE66-41)
Product Specific Notices for TFIIE-alpha Antibody (JE66-41)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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