TFIIE-alpha Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46195
![Immunohistochemistry-Paraffin: TFIIE-alpha Antibody [NBP1-46195]](https://resources.bio-techne.com/images/products/TFIIE-alpha-Antibody-Immunohistochemistry-NBP1-46195-img0003.jpg "Immunohistochemistry-Paraffin: TFIIE-alpha Antibody [NBP1-46195]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Primate (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 100 and 150 of human General Transcription Factor IIE, polypeptide 1, alpha, using the numbering given in entry NP_005504.2 (GeneID 2960).
Reactivity Notes
Orangutan (100%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TFIIE-alpha Antibody
Immunohistochemistry-Paraffin: TFIIE-alpha Antibody [NBP1-46195]
Immunohistochemistry-Paraffin: TFIIE-alpha Antibody [NBP1-46195] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- GTF2E1/TFIIE-alpha used at a dilution of 1:1,000 (1ug/ml). Detection: DAB![Immunoprecipitation: TFIIE-alpha Antibody [NBP1-46195]](https://resources.bio-techne.com/images/products/TFIIE-alpha-Antibody-Immunoprecipitation-NBP1-46195-img0002.jpg "Immunoprecipitation: TFIIE-alpha Antibody [NBP1-46195]")
Immunoprecipitation: TFIIE-alpha Antibody [NBP1-46195]
Immunoprecipitation: TFIIE-alpha Antibody [NBP1-46195] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-GTF2E1/TFIIE-alpha antibody used for IP at 3 mcg/mg lysate. GTF2E1/TFIIE-alpha was also immunoprecipitated by rabbit anti-GTF2E1/TFIIE-alpha antibody NBP1-46196, which recognizes a downstream epitope.Applications for TFIIE-alpha Antibody
Application
Recommended Usage
Immunohistochemistry
1:500-1:2000
Immunohistochemistry-Paraffin
1:500-1:2000
Immunoprecipitation
2-5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: TFIIE-alpha
Alternate Names
FE, General transcription factor IIE 56 kDa subunit, general transcription factor IIE subunit 1, general transcription factor IIE, polypeptide 1 (alpha subunit, 56kD), general transcription factor IIE, polypeptide 1, alpha 56kDa, TF2E1, TFIIE-A, TFIIE-alpha, Transcription initiation factor IIE subunit alpha
Gene Symbol
GTF2E1
Additional TFIIE-alpha Products
Product Documents for TFIIE-alpha Antibody
Product Specific Notices for TFIIE-alpha Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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