TGF-beta 2 Antibody

Detection of Human TGF-beta 2 Antibody by Western Blot

MiR-30b induces expression of TGF beta2.(A) HUVECs were transfected with either control mimic (con) or miR-30b mimic (30b) and levels of TGF beta1 and TGF beta2 mRNA were assessed by qRT-PCR. Expression levels relative to control mimic transfected cells and normalized to beta-actin expression are presented as the mean ± SEM (n = 2). Overexpression of miR-30b significantly increases TGF beta2 expression. * P < 0.05, ** P < 0.01 as determined by unpaired Student’s t-test. (B) Cells were transfected with 20 nM of either control mimic (control) or miR-30b mimic (miR-30b) and protein lysates were collected after 48 hours for assessment of TGF beta2 protein levels by western blot. beta-actin was used as endogenous control. (C) ELISAs for TGF beta1 and TGF beta2 were performed with 24 hour conditioned supernates from HUVECs transfected with 20 nM of either control or miR-30b mimic. Data represents the mean ± SEM (n = 2). Overexpression of miR-30b significantly increases TGF beta2 secretion into cell culture supernate. * P = 0.044 as determined by unpaired Student’s t-test. (D) HUVECs were transfected with 20 nM of either control mimic (control) or miR-30b mimic (miR-30b) and protein lysates were collected after 48 hours for assessment of Smad2 phosphorylation by western blot. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28977001), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human TGF-beta 2 Antibody by Western Blot

MiR-30b induces expression of TGF beta2.(A) HUVECs were transfected with either control mimic (con) or miR-30b mimic (30b) and levels of TGF beta1 and TGF beta2 mRNA were assessed by qRT-PCR. Expression levels relative to control mimic transfected cells and normalized to beta-actin expression are presented as the mean ± SEM (n = 2). Overexpression of miR-30b significantly increases TGF beta2 expression. * P < 0.05, ** P < 0.01 as determined by unpaired Student’s t-test. (B) Cells were transfected with 20 nM of either control mimic (control) or miR-30b mimic (miR-30b) and protein lysates were collected after 48 hours for assessment of TGF beta2 protein levels by western blot. beta-actin was used as endogenous control. (C) ELISAs for TGF beta1 and TGF beta2 were performed with 24 hour conditioned supernates from HUVECs transfected with 20 nM of either control or miR-30b mimic. Data represents the mean ± SEM (n = 2). Overexpression of miR-30b significantly increases TGF beta2 secretion into cell culture supernate. * P = 0.044 as determined by unpaired Student’s t-test. (D) HUVECs were transfected with 20 nM of either control mimic (control) or miR-30b mimic (miR-30b) and protein lysates were collected after 48 hours for assessment of Smad2 phosphorylation by western blot. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28977001), licensed under a CC-BY license. Not internally tested by R&D Systems.