TGR5/GPBAR1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TGR5/GPBAR1 Antibody - BSA Free [NBP2-23669]

Immunocytochemistry/Immunofluorescence: TGR5/GPBAR1 Antibody [NBP2-23669] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-TGR5/GPBAR1 at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Knockdown Validated: TGR5/GPBAR1 Antibody - BSA Free [NBP2-23669]

Knockdown Validated: TGR5/GPBAR1 Antibody [NBP2-23669] - LCA-induced oxidative stress responses were mediated by TGR5/GPBAR1 and by CAR receptors. TGR5/GPBAR1, CAR, VDR, and PXR bile acid receptors were silenced in MCF7 cells by transiently transfecting the cells with the corresponding siRNA or a negative control siRNA. After 48 h, protein expressions of NRF2 and iNOS were determined by western blotting (n = 3). Data are plotted as mean +/- SEM. * p < 0.05, control vs. LCA/siRNA treated. (CAR, constitutive androstane receptor; FXR, farnesoid X-activated receptor; LCA, lithocholic acid; LXR, liver X nuclear receptor; NRF2, nuclear factor, erythroid 2-like 2; TGR5/GPBAR1, G protein-coupled bile acid receptor 1/Takeda G-protein coupled receptor; VDR, vitamin D receptor). Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/2072-6694/11/9/1255), licensed under a CC-BY license.

Western Blot: TGR5/GPBAR1 Antibody - BSA Free [NBP2-23669] -

Western Blot: TGR5/GPBAR1 Antibody - BSA Free [NBP2-23669] - LCA-induced oxidative stress responses were mediated by TGR5 & by CAR receptors. (A) The 4T1 cells were treated with 0.3 μm LCA & NF449, CINPA1, DY268, or GSK2033 at a final concentration of 5 μm for 48 h, then NRF2 protein expression was detected using western blotting (representative figure, n = 2). (B,C) TGR5, CAR, VDR, & PXR bile acid receptors were silenced in MCF7 cells by transiently transfecting the cells with the corresponding siRNA or a negative control siRNA. After 48 h, protein expressions of (B,C) NRF2 & (C) iNOS were determined by western blotting (n = 3). Data are plotted as mean ± SEM. * p < 0.05, control vs. LCA/siRNA treated. (CAR, constitutive androstane receptor; FXR, farnesoid X-activated receptor; LCA, lithocholic acid; LXR, liver X nuclear receptor; NRF2, nuclear factor, erythroid 2-like 2; TGR5/GPBAR1, G protein-coupled bile acid receptor 1/Takeda G-protein coupled receptor; VDR, vitamin D receptor). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31461945), licensed under a CC-BY license. Not internally tested by Novus Biologicals.