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THAP1 Antibody (2C1-2F2)

Novus Biologicals, part of Bio-Techne | Catalog # H00055145-M01

Novus Biologicals, part of Bio-Techne
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H00055145-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2C1-2F2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

THAP1 (AAH21721, 1 a.a. ~ 213 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVQSCSAYGCKNRYDKDKPVSFHKFPLTRPSLCKEWEAAVRRKNFKPTKYSSICSEHFTPDCFKRECNNKLLKENAVPTIFLCTEPHDKKEDLLEPQEQLPPPPLPPPVSQVDAAIGLLMPPLQTPVNLSVFCDHNYTVEDTMHQRKRIHQLEQQVEKLRKKLKTAQQRCRRQERQLEKLKEVVHFQKEKDDVSERGYVILPNDYFEIVEVPA

Reactivity Notes

Human. Other species not tested.

Specificity

THAP1 - THAP domain containing, apoptosis associated protein 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for THAP1 Antibody (2C1-2F2)

Western Blot: THAP1 Antibody (2C1-2F2) [H00055145-M01]

Western Blot: THAP1 Antibody (2C1-2F2) [H00055145-M01]

Western Blot: THAP1 Antibody (2C1-2F2) [H00055145-M01] - Analysis of THAP1 over-expressed 293 cell line, cotransfected with THAP1 Validated Chimera RNAi ( Cat # H00055145-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with THAP1 monoclonal antibody (M01), clone 2C1-2F2 (Cat # H00055145-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunocytochemistry/ Immunofluorescence: THAP1 Antibody (2C1-2F2) [H00055145-M01]

Immunocytochemistry/ Immunofluorescence: THAP1 Antibody (2C1-2F2) [H00055145-M01]

Immunocytochemistry/Immunofluorescence: THAP1 Antibody (2C1-2F2) [H00055145-M01] - Analysis of monoclonal antibody to THAP1 on HeLa cell . Antibody concentration 10 ug/ml.
Western Blot: THAP1 Antibody (2C1-2F2) [H00055145-M01]

Western Blot: THAP1 Antibody (2C1-2F2) [H00055145-M01]

Western Blot: THAP1 Antibody (2C1-2F2) [H00055145-M01] - Analysis of THAP1 expression in transfected 293T cell line by THAP1 monoclonal antibody (M01), clone 2C1-2F2.Lane 1: THAP1 transfected lysate(24.9 KDa).Lane 2: Non-transfected lysate.

Applications for THAP1 Antibody (2C1-2F2)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. It has also been used for RNAi Validation.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: THAP1

The protein encoded by this gene contains a THAP domain, a conserved DNA-binding domain. This protein colocalizes with the apoptosis response protein PAWR/PAR-4 in promyelocytic leukemia (PML) nuclear bodies, and functions as a proapoptotic factor that links PAWR to PML nuclear bodies. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Alternate Names

4833431A01Rik, dystonia 6, torsion (autosomal dominant), DYT6, FLJ10477, MGC33014, nuclear proapoptotic factor, THAP domain containing, apoptosis associated protein 1, THAP domain protein 1, THAP domain-containing protein 1

Entrez Gene IDs

55145 (Human)

Gene Symbol

THAP1

OMIM

609520 (Human)

Additional THAP1 Products

Product Documents for THAP1 Antibody (2C1-2F2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for THAP1 Antibody (2C1-2F2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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