THEM2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92498

Novus Biologicals, part of Bio-Techne
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NBP1-92498
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all THEM2 Antibodies »

Product Summary for THEM2 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GAPGVSVDMNITYMSPAKLGEDIVITAHVLKQGKTLAFTSVDLTNKATGKLIAQGRHTKHLGN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for THEM2 Antibody

Western Blot: THEM2 Antibody [NBP1-92498]

Western Blot: THEM2 Antibody [NBP1-92498]

Western Blot: THEM2 Antibody [NBP1-92498] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/ Immunofluorescence: THEM2 Antibody [NBP1-92498]

Immunocytochemistry/ Immunofluorescence: THEM2 Antibody [NBP1-92498]

Immunocytochemistry/Immunofluorescence: THEM2 Antibody [NBP1-92498] - Immunofluorescent staining of human cell line A-431 shows localization to cell junctions.
Immunohistochemistry-Paraffin: THEM2 Antibody [NBP1-92498]

Immunohistochemistry-Paraffin: THEM2 Antibody [NBP1-92498]

Immunohistochemistry-Paraffin: THEM2 Antibody [NBP1-92498] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
View 1 more images

Applications for THEM2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: THEM2

THEM2 encodes a member of the thioesterase superfamily. In humans, the protein co-localizes with microtubules and is essential for sustained cell proliferation. The orthologous mouse protein forms a homotetramer and is associated with mitochondria. The mouse protein functions as a medium- and long-chain acyl-CoA thioesterase. Multiple transcript variants encoding different isoforms have been found for this gene.

Alternate Names

acyl-CoA thioesterase 13PNAS-27, EC 3.1.2.-, HT012, hypothalamus protein HT012, MGC4961, THEM2, Thioesterase superfamily member 2acyl-coenzyme A thioesterase 13

Gene Symbol

ACOT13

Additional THEM2 Products

Product Documents for THEM2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for THEM2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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