Thioredoxin-2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00013

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Thioredoxin-2 Antibodies »

Product Summary for Thioredoxin-2 Antibody

Immunogen

Produced in rabbits immunized with purified, recombinant Human Thioredoxin-2 (Uniprot#: Q99757; Thr60-Gly166)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Thioredoxin-2 Antibody

Western Blot: Thioredoxin-2 Antibody [NBP3-00013]

Western Blot: Thioredoxin-2 Antibody [NBP3-00013]

Western Blot: Thioredoxin-2 Antibody [NBP3-00013] - Anti-Thioredoxin-2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: 293 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa
Immunocytochemistry/ Immunofluorescence: Thioredoxin-2 Antibody [NBP3-00013]

Immunocytochemistry/ Immunofluorescence: Thioredoxin-2 Antibody [NBP3-00013]

Immunocytochemistry/Immunofluorescence: Thioredoxin-2 Antibody [NBP3-00013] - Immunofluorescence staining of Thioredoxin-2 in HELA cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Thioredoxin-2 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
Immunohistochemistry-Paraffin: Thioredoxin-2 Antibody [NBP3-00013]

Immunohistochemistry-Paraffin: Thioredoxin-2 Antibody [NBP3-00013]

Immunohistochemistry-Paraffin: Thioredoxin-2 Antibody [NBP3-00013] - Immunochemical staining of human Thioredoxin-2 in human small intestine with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
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Applications for Thioredoxin-2 Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

5-10 uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Thioredoxin-2

This nuclear gene encodes a mitochondrial member of the thioredoxin family, a group of small multifunctional redox-active proteins. The encoded protein may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis. (provided by RefSeq)

Alternate Names

MT-TRX, Thioredoxin2, Trx2, TXN2

Gene Symbol

TXN2

Additional Thioredoxin-2 Products

Product Documents for Thioredoxin-2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Thioredoxin-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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