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THRAP3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86915

Novus Biologicals, part of Bio-Techne
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NBP1-86915
NBP1-86915-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for THRAP3 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ETEEREESTTGFDKSRLGTKDFVGPSERGGGRARGTFQFRARGRGWGRGNYSGNNNNNSNNDFQKRNREEEWDPEYTPKSKKYYLHDDREGEGSDKWVSRGRGRGAFPRGRGRFMFRKSSTSPKWAHDKFS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for THRAP3 Antibody

Western Blot: THRAP3 Antibody [NBP1-86915]

Western Blot: THRAP3 Antibody [NBP1-86915]

Western Blot: THRAP3 Antibody [NBP1-86915] - Analysis in human cell line RT-4 and human cell line U-251 MG.
Immunocytochemistry/ Immunofluorescence: THRAP3 Antibody [NBP1-86915]

Immunocytochemistry/ Immunofluorescence: THRAP3 Antibody [NBP1-86915]

Immunocytochemistry/Immunofluorescence: THRAP3 Antibody [NBP1-86915] - Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.
Immunohistochemistry-Paraffin: THRAP3 Antibody [NBP1-86915]

Immunohistochemistry-Paraffin: THRAP3 Antibody [NBP1-86915]

Immunohistochemistry-Paraffin: THRAP3 Antibody [NBP1-86915] - Staining of human tonsil shows strong nuclear positivity in both germinal and non-germinal center cells.

Applications for THRAP3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: THRAP3

Thyroid hormone receptors (TRs) are ligand dependent members of the steroid/retinoic acid superfamily of transcription factors. The two genes encoding TRs identified to date, TR alpha and TR beta, have been mapped to human chromosomes 17 and 3, respectively. TRs bind to thyroid hormone response elements (TREs) with half site binding motifs in the orientation of palindromes, direct repeats, or inverted palindromes. The affinities of binding are both variable and influenced differentially by 3,5,3 triiodo L thyronine (T3). Thyroid hormones, through their interaction with the thyroid receptor (TR), effect metabolic processes, growth and development in many tissues by regulating the expression of genes for growth hormone, malic enzyme and several hepatic proteins.

Alternate Names

FLJ22082, MGC133082, MGC133083, thyroid hormone receptor associated protein 3, Thyroid hormone receptor-associated protein complex 150 kDa component, thyroid hormone receptor-associated protein, 150 kDa subunit, Trap150, TRAP150thyroid hormone receptor-associated protein 3

Gene Symbol

THRAP3

Additional THRAP3 Products

Product Documents for THRAP3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for THRAP3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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