Thrombopoietin R/Tpo R Antibody (S03-1C9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19792

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S03-1C9

Concentration

0.3 mg/ml

View all Thrombopoietin R/Tpo R Antibodies »

Product Summary for Thrombopoietin R/Tpo R Antibody (S03-1C9)

Immunogen

A synthetic peptide of human Thrombopoietin R/Tpo R (Uniprot # P40238)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Thrombopoietin R/Tpo R Antibody (S03-1C9)

Western Blot: Thrombopoietin R/Tpo R Antibody (S03-1C9) [NBP3-19792]

Western Blot: Thrombopoietin R/Tpo R Antibody (S03-1C9) [NBP3-19792]

Western Blot: Thrombopoietin R/Tpo R Antibody (S03-1C9) [NBP3-19792] - Western blot detection of Thrombopoietin R/Tpo R in K562, C6 cell lysates using NBP3-19792 (1:1000 diluted). Predicted band size: 71kDa. Observed band size: 85kDa.

Applications for Thrombopoietin R/Tpo R Antibody (S03-1C9)

Application
Recommended Usage

Western Blot

1/1000-1/5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Thrombopoietin R/Tpo R

In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs . TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated. [provided by RefSeq]

Long Name

Thrombopoietin Receptor

Alternate Names

c-Mpl, CD110, MPL, MPLV, TpoR

Gene Symbol

MPL

Additional Thrombopoietin R/Tpo R Products

Product Documents for Thrombopoietin R/Tpo R Antibody (S03-1C9)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Thrombopoietin R/Tpo R Antibody (S03-1C9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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