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Thrombospondin-1 Antibody (A6.1)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2059

Novus Biologicals, part of Bio-Techne
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NB100-2059
NB100-2059SS

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat

Applications

Validated:

Block/Neutralize, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Block/Neutralize, IF/IHC, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, PCR, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # A6.1

Concentration

0.2 mg/ml

Product Specifications

Immunogen

BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.

Localization

Secretory granules, Golgi complex, endoplasmic reticulum

Specificity

This is specific to 450 kD protein, a non-reduced form, and 170 to 180 kD protein, a reduced form, of Thrombospondin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Thrombospondin-1 Antibody (A6.1)

Immunohistochemistry: Thrombospondin-1 Antibody (A6.1) [NB100-2059]

Immunohistochemistry: Thrombospondin-1 Antibody (A6.1) [NB100-2059]

Immunohistochemistry: Thrombospondin-1 Antibody (A6.1) [NB100-2059] - Representative photomicrographs showing Thrombospondin-1 (A) immunostaining in duodenal sections of 1 non-CD controls (NC) and 1 active celiac disease (ACD) patient. Isotype control staining is also shown. Right panels: positive cells were counted in at least 5 fields/section of duodenal samples of 5 NC and 5 ACD patients and expressed as mean +/- SD of all experiments. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0100980), licensed under a CC-BY license.
Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059]

Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059]

Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059] - Staining in human placenta.
Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059]

Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059]

Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059] - Ang II increases the number of TSP-1 positive cells in mouse heart and skin.IHC staining of TSP-1 was performed on paraffin sections from the skin of PBS and Ang II treated WT mice. Representative photographs are shown from five animals per group. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0109763), licensed under a CC-BY license.

Applications for Thrombospondin-1 Antibody (A6.1)

Application
Recommended Usage

Immunohistochemistry

1:25-1:50

Immunohistochemistry-Paraffin

1:25-1:50
Application Notes
IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT. Use in Blocking/Neutralizing reported in scientific literature (PMID: 19509293). Use in Western blot reported in reported in scientific literature (PMID: 27447109). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 24971453).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.4), 0.2% BSA, Tween-20

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Thrombospondin-1

Thrombospondin is a protein from platelet a granules. It is secreted at sites of platelet activation and aggregation and is involved in the differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.

Alternate Names

THBS1, Thrombospondin1, TSP-1

Entrez Gene IDs

7057 (Human); 56229 (Mouse); 364630 (Rat)

Gene Symbol

THBS1

UniProt

Additional Thrombospondin-1 Products

Product Documents for Thrombospondin-1 Antibody (A6.1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Thrombospondin-1 Antibody (A6.1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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