Thymidylate Synthase Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB600-642
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Format
BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Thymidylate Synthase Antibody was purified from rabbit serum after repeated immunizations with recombinant human Thymidylate Synthase (36 kDa) produced in E.coli. (Uniprot: P04818)
Reactivity Notes
In general, this antibody can detect antigen in a variety of human cells and tissues, as well as bacteria, African green monkey, rat and mouse. Somewhat lower dilutions may be required in some non-human cell lines.
Specificity
Anti-THYMIDYLATE SYNTHASE IgG fraction is directed against human Thymidylate Synthase and is useful in determining its presence in various assays. Because inhibition of Thymidylate Synthase prevents DNA synthesis and cell proliferation, the enzyme is an important target for cancer chemotherapeutic drugs, specifically the fluoropyrimidine group of antineoplastic drugs used to treat solid tumors. In general, this antibody can detect antigen in a variety of human cells and tissues, as well as bacteria, African green monkey, rat and mouse. Somewhat lower dilutions may be required in some non-human cell lines. Anti-Thymidylate Synthase can detect Thymidylate Synthase by immunochemistry in proliferating cell cultures and tissues but does not stain nonproliferating cells. Normal colon mucosa shows weak staining; however, some colorectal cancer specimens show very strong staining.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Description
This antibody IgG fraction is directed against human Thymidylate Synthase and is useful in determining its presence in various assays
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Thymidylate Synthase Antibody - BSA Free
![Western Blot: Thymidylate Synthase AntibodyBSA Free [NB600-642]](https://resources.bio-techne.com/images/products/Thymidylate-Synthase-Antibody-Western-Blot-NB600-642-img0003.jpg "Western Blot: Thymidylate Synthase AntibodyBSA Free [NB600-642]")
Western Blot: Thymidylate Synthase AntibodyBSA Free [NB600-642]
Western Blot: Thymidylate Synthase Antibody [NB600-642] - Detection thymidylate synthase present in a HeLa cell extract. Each lane is estimated to contain 4 ug of protein. Lanes 1, 2 and 3 represent 1:2,000, 1:5,000 and 1:10,000 fold dilutions of the antibody. Detection was made using HRP Rabbit-a-Sheep IgG (613-4302) diluted 1:1,000 and color development using TMB substrate for approximately 4'.![Western Blot: Thymidylate Synthase AntibodyBSA Free [NB600-642]](https://resources.bio-techne.com/images/products/Thymidylate-Synthase-Antibody-Western-Blot-NB600-642-img0004.jpg "Western Blot: Thymidylate Synthase AntibodyBSA Free [NB600-642]")
Western Blot: Thymidylate Synthase AntibodyBSA Free [NB600-642]
Western Blot: Thymidylate Synthase Antibody [NB600-642] - Detection thymidylate synthase present in a HeLa cell extract. Each lane is estimated to contain 4 ug of protein. Lanes 1, 2 and 3 represent 1:2,000, 1:5,000 and 1:10,000 fold dilutions of the antibody. Detection was made using HRP Rabbit-a-Sheep IgG (613-4302) diluted 1:1,000 and color development using TMB substrate for approximately 4'.Applications for Thymidylate Synthase Antibody - BSA Free
Application
Recommended Usage
ELISA
1:10000-1:50000
Immunocytochemistry/ Immunofluorescence
1:10 - 1:500
Immunohistochemistry
1:10 - 1:500
Immunohistochemistry-Paraffin
1:10 - 1:500
Western Blot
1:500-1:2000
Application Notes
This product has been tested by western blot. This antibody is suitable for use in ELISA, immunoprecipitation, immunofluorescence microscopy, and immunohistochemistry. The antibody recognizes the expected additional band corresponding to the ternary complex of hTS-dFUMP-reduced folate in HeLa cells treated with the TS inhibitor 5-FUdR. This event occurs in most human breast, colorectal, gastric, head and neck carcinomas. The antibody recognizes the 36,000 MW hTS. Reactivity in other immunoassays is unknown.
Formulation, Preparation, and Storage
Purification
IgG purified
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
BSA Free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Thymidylate Synthase
Long Name
Thymidylate synthase
Alternate Names
EC 2.1.1.45, OK/SW-cl.29, TS, TSase, TYMS
Gene Symbol
TYMS
UniProt
Additional Thymidylate Synthase Products
Product Documents for Thymidylate Synthase Antibody - BSA Free
Product Specific Notices for Thymidylate Synthase Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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