Thymidylate Synthase Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99086
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Thymidylate Synthase.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Thymidylate Synthase Antibody
Western Blot: Thymidylate Synthase Antibody [NBP2-99086]
Western Blot: Thymidylate Synthase Antibody [NBP2-99086] - Anti-Thymidylate Synthase rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: HL60 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 36 kDa (We are unsure as to the identity of these extra bands).Immunoprecipitation: Thymidylate Synthase Antibody [NBP2-99086]
Immunoprecipitation: Thymidylate Synthase Antibody [NBP2-99086] - Thymidylate Synthase was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate4 ul anti-Thymidylate Synthase rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Thymidylate Synthase rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 36 kDaApplications for Thymidylate Synthase Antibody
Application
Recommended Usage
Immunoprecipitation
4-8 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Thymidylate Synthase
Long Name
Thymidylate synthase
Alternate Names
EC 2.1.1.45, OK/SW-cl.29, TS, TSase, TYMS
Gene Symbol
TYMS
Additional Thymidylate Synthase Products
Product Documents for Thymidylate Synthase Antibody
Product Specific Notices for Thymidylate Synthase Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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