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Thymidylate Synthase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99086

Novus Biologicals, part of Bio-Techne
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NBP2-99086-100ul
NBP2-99086-50ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Thymidylate Synthase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Thymidylate Synthase Antibody

Western Blot: Thymidylate Synthase Antibody [NBP2-99086]

Western Blot: Thymidylate Synthase Antibody [NBP2-99086]

Western Blot: Thymidylate Synthase Antibody [NBP2-99086] - Anti-Thymidylate Synthase rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: HL60 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 36 kDa (We are unsure as to the identity of these extra bands).
Immunoprecipitation: Thymidylate Synthase Antibody [NBP2-99086]

Immunoprecipitation: Thymidylate Synthase Antibody [NBP2-99086]

Immunoprecipitation: Thymidylate Synthase Antibody [NBP2-99086] - Thymidylate Synthase was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate4 ul anti-Thymidylate Synthase rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Thymidylate Synthase rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 36 kDa

Applications for Thymidylate Synthase Antibody

Application
Recommended Usage

Immunoprecipitation

4-8 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Thymidylate Synthase

Thymidylate synthase (TS) is an intracellular enzyme critical for de novo synthesis of DNA. In cancer, expression of TS is often elevated and becomes further elevated as a result of treatment with the most commonly used chemotherapeutic, 5-fluorouracil (5-FU). Resistance or lack of response to 5-FU is attributed to the elevation of TS activity. Overexpression of TS has been demonstrated in many types of tumors including breast, colon, gastric and melanoma and is usually predictive of shortened survival time.

Long Name

Thymidylate synthase

Alternate Names

EC 2.1.1.45, OK/SW-cl.29, TS, TSase, TYMS

Gene Symbol

TYMS

Additional Thymidylate Synthase Products

Product Documents for Thymidylate Synthase Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Thymidylate Synthase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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