Thyroglobulin Antibody (SPM221) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34748
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
CyTOF-ready, ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM221
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Human thyroid follicular cells
Localization
Cytoplasmic and secreted
Specificity
Thyroglobulin is a 660kDa dimeric pre-protein with multiple glycosylation sites. It is produced by and processed within the thyroid gland to produce the hormone thyroxine and triiodothyronine. Prior to forming dimers, thyroglobulin monomers undergo conformational maturation in the endoplasmic reticulation. The vast majority of follicular carcinomas of the thyroid will give positive immunoreactivity for anti-thyroglobulin even though sometimes only focally. Poorly differentiated carcinomas of the thyroid are frequently anti-thyroglobulin negative. Adenocarcinomas of other-than-thyroid origin do not react with this antibody. This antibody is useful in identification of thyroid carcinoma of the papillary and follicular types. Presence of thyroglobulin in metastatic lesions establishes the thyroid origin of tumor. Anti-thyroglobulin, combined with anti-calcitonin, can identify medullary carcinomas of the thyroid. Furthermore, anti-thyroglobulin, combined with anti-TTF1, can be a reliable marker to differentiate between primary thyroid and lung neoplasms.
Marker
Thyroidal Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32904).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Thyroglobulin Antibody (SPM221) - Azide and BSA Free
Immunohistochemistry-Paraffin: Thyroglobulin Antibody (SPM221) - Azide and BSA Free [NBP2-34748]
Immunohistochemistry-Paraffin: Thyroglobulin Antibody (SPM221) - Azide and BSA Free [NBP2-34748] - Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with Thyroglobulin Antibody (SPM221).Applications for Thyroglobulin Antibody (SPM221) - Azide and BSA Free
Application
Recommended Usage
ELISA
1-5ug/ml for coating
Flow Cytometry
0.5-1ug/million cells
Immunohistochemistry-Paraffin
0.5-1ug/ml
Western Blot
0.5-1ug/ml
Application Notes
ELISA: For coating, order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Thyroglobulin
Alternate Names
AITD3, cog, TDH3, Tg, TGN
Gene Symbol
TG
Additional Thyroglobulin Products
Product Documents for Thyroglobulin Antibody (SPM221) - Azide and BSA Free
Product Specific Notices for Thyroglobulin Antibody (SPM221) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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