Thyroid receptor-interacting protein 12 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-97822
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 75 and 125 of human thyroid hormone receptor interactor 12 using the numbering given in entry NP_004229.1 (GeneID 9320).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Thyroid receptor-interacting protein 12 Antibody
Western Blot: Thyroid receptor-interacting protein 12 Antibody [NB100-97822]
Western Blot: Thyroid receptor-interacting protein 12 Antibody [NB100-97822] - Detection of human TRIP12 by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIP12 antibody NB100-97822 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Immunoprecipitation: Thyroid receptor-interacting protein 12 Antibody [NB100-97822]
Immunoprecipitation: Thyroid receptor-interacting protein 12 Antibody [NB100-97822] - Detection of human TRIP12 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRIP12 antibody A301-814A (lot A301-814A-2) used for IP at 6 ug per reaction. TRIP12 was also immunoprecipitated by a previous lot of this antibody (lot A301-814A-1). For blotting immunoprecipitated TRIP12, A301-814A was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for Thyroid receptor-interacting protein 12 Antibody
Application
Recommended Usage
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:10000 -1:25000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Thyroid receptor-interacting protein 12
Alternate Names
EC 6.3.2.-, KIAA0045ULF, MGC138849, MGC138850, probable E3 ubiquitin-protein ligase TRIP12, thyroid hormone receptor interactor 12, thyroid receptor interacting protein 12, Thyroid receptor-interacting protein 12, TR-interacting protein 12, TRIP-12
Entrez Gene IDs
9320 (Human)
Gene Symbol
TRIP12
UniProt
Additional Thyroid receptor-interacting protein 12 Products
Product Documents for Thyroid receptor-interacting protein 12 Antibody
Product Specific Notices for Thyroid receptor-interacting protein 12 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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