Thyrotropin Releasing Hormone Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-95244
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 25-230 of human Thyrotropin Releasing Hormone (NP_009048.1). QPEAAQQEAVTAAEHPGLDDFLRQVERLLFLRENIQRLQGDQGEHSASQIFQSDWLSKRQHPGKREEEEEEGVEEEEEEEGGAVGPHKRQHPGRREDEASWSVDVTQHKRQHPGRRSPWLAYAVPKRQHPGRRLADPKAQRSWEEEEEEEEREEDLMPEKRQHPGKRALGGPCGPQGAYGQAGLLLGLLDDLSRSQGAEEKRQHPG
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Thyrotropin Releasing Hormone Antibody - Azide and BSA Free
![Western Blot: Thyrotropin Releasing Hormone AntibodyAzide and BSA Free [NBP2-95244]](https://resources.bio-techne.com/images/products/Thyrotropin-Releasing-Hormone-Antibody-Western-Blot-NBP2-95244-img0004.jpg "Western Blot: Thyrotropin Releasing Hormone AntibodyAzide and BSA Free [NBP2-95244]")
Western Blot: Thyrotropin Releasing Hormone AntibodyAzide and BSA Free [NBP2-95244]
Western Blot: Thyrotropin Releasing Hormone Antibody [NBP2-95244] - Analysis of extracts of various cell lines, using Thyrotropin Releasing Hormone at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.
Immunohistochemistry:Thyrotropin Releasing Hormone Antibody [NBP2-95244] -
Immunohistochemistry:Thyrotropin Releasing Hormone Antibody [NBP2-95244] - Analysis of Mouse pituitary gland tissue using TRH Rabbit pAb at a dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.Applications for Thyrotropin Releasing Hormone Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100 - 1:500
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Thyrotropin Releasing Hormone
Alternate Names
MGC125964, MGC125965, prothyroliberin, thyrotropin-releasing hormone
Gene Symbol
TRH
Additional Thyrotropin Releasing Hormone Products
Product Documents for Thyrotropin Releasing Hormone Antibody - Azide and BSA Free
Product Specific Notices for Thyrotropin Releasing Hormone Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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