Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all TIAL1 Antibodies »

Product Summary for TIAL1 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 325 and 375 of human TIA-1-Related Protein using the numbering given in entry NP_003243.1 (GeneID 7073).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TIAL1 Antibody

Western Blot: TIAL1 Antibody [NBP2-04047]

Western Blot: TIAL1 Antibody [NBP2-04047]

Western Blot: TIAL1 Antibody [NBP2-04047] - Detection of human TIAR by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TIAR antibody NBP2-04047 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. TIAR was also immunoprecipitated by another rabbit anti-TIAR antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Immunoprecipitation: TIAL1 Antibody [NBP2-04047]

Immunoprecipitation: TIAL1 Antibody [NBP2-04047]

Immunoprecipitation: TIAL1 Antibody [NBP2-04047] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TIAR antibodyNBP1-97738 used for IP at 6 ug/mg lysate. TIAR was also immunoprecipitated by rabbit anti-TIAR antibody NBP2-04047, which recognizes a downstream epitope. For blotting immunoprecipitated TIAR, NBP2-04047 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for TIAL1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 mcg/mg

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TIAL1

TIAL1 is encoded by this gene is a member of a family of RNA-binding proteins, has three RNA recognition motifs (RRMs), and binds adenine and uridine-rich elements in mRNA and pre-mRNAs of a wide range of genes. It regulates various activities including translational control, splicing and apoptosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The different isoforms have been show to function differently with respect to post-transcriptional silencing.

Alternate Names

aging-associated gene 7 protein, MGC33401, TCBP, T-cluster binding protein, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TIA1 cytotoxic granule-associated RNA-binding protein-like 1, TIA-1 related protein, TIA-1-related nucleolysin, TIA-1-related protein, TIARnucleolysin TIAR

Gene Symbol

TIAL1

Additional TIAL1 Products

Product Documents for TIAL1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TIAL1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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