Western Blot: Tie-1 Antibody (Cl. 6F12) [NBP1-18610] - Analysis of recombinant human and mouse soluble TIEreceptors using a monoclonal antibody directed against human recombinant soluble TIE-1. There is a weak cross reactivity with human sTIE-2 but no reactivity with mouse sTIE-1 and sTIE-2 visible.

Flow Cytometry: Tie-1 Antibody (6F12) - Azide and BSA Free [NBP1-18610]

Flow Cytometry: Tie-1 Antibody (Cl. 6F12) [NBP1-18610] - Fig. 1: Flow analysis of TIE-1 expression in primary human umbilical vein endothelial cells (HUVEC).

Applications for Tie-1 Antibody (6F12) - Azide and BSA Free

Application

Recommended Usage

ELISA

1 - 15 ug/ml

Western Blot

1 - 2 ug/ml

Application Notes

This antibody is Cytof ready.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Please note the sample size of this product will be provided in reconstituted liquid form.

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tie-1

TIE1/TIE (tyrosine kinase with Ig and EGF homology domains 1) and TIE2/Tek define a new class of the receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin like domains flanking three epidermal growth factor (EFG) like domains followed by three fibronectin type III like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. Human TIE1 cDNA encodes a 1138 amino acid residue precursor protein with a putative signal peptide, an extracellular domain, and a cytoplasmic domain. Human TIE1/Fc, a disulfide linked homodimeric protein, has a calculated molecular mass of approximately 107 kDa. Due to glycosylation, the protein migrates to approximately 160 kDa in SDS PAGE under reducing conditions. TIE1 and TIE2, expressed primarily on endothelial and hematopoietic progenitor cells, play important roles in angiogenesis, vasculogenesis, and hematopoiesis. In developing vascular endothelial cells, TIE1 and TIE2 are specifically expressed. Two ligands that bind TIE have been identified, angiopoietin 1 and angiopoietin 2. Based on gene targeting studies, the in vivo functions of TIE1 are related to endothelial cell differentiation. The receptor tyrosine kinase TIE also plays a role in the survival and integrity of the endothelium.

Long Name

Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 1

Alternate Names

Tie1

Entrez Gene IDs

7075 (Human)

Gene Symbol

TIE1

UniProt

P35590

Additional Tie-1 Products

Product Documents for Tie-1 Antibody (6F12) - Azide and BSA Free

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Tie-1 Antibody (6F12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Calculators
Flow Cytometry

Tie-1 Antibody (6F12) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-18610

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

Format

Concentration

View all Tie-1 Antibodies »

Product Summary for Tie-1 Antibody (6F12) - Azide and BSA Free

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Tie-1 Antibody (6F12) - Azide and BSA Free

Western Blot: Tie-1 Antibody (6F12)Azide and BSA Free [NBP1-18610]

Flow Cytometry: Tie-1 Antibody (6F12) - Azide and BSA Free [NBP1-18610]

Applications for Tie-1 Antibody (6F12) - Azide and BSA Free

ELISA

Western Blot

Formulation, Preparation, and Storage

Purification

Reconstitution

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: Tie-1

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional Tie-1 Products

Product Documents for Tie-1 Antibody (6F12) - Azide and BSA Free

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Tie-1 Antibody (6F12) - Azide and BSA Free