Tie-2 Antibody (MM0018-21G7) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-60986

Novus Biologicals, part of Bio-Techne
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NB110-60986
NB110-60986-0.025mg
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MM0018-21G7

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Tie-2 Antibodies »

Product Specifications

Immunogen

This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with purified human extracellular domain of Tie2 fragment).

Specificity

Recognizes human Tie2, no cross-reactivity with human Tie1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Tie-2 Antibody (MM0018-21G7) - Azide and BSA Free

Western Blot: Tie-2 Antibody (MM0018-21G7)Azide and BSA Free [NB110-60986]

Western Blot: Tie-2 Antibody (MM0018-21G7)Azide and BSA Free [NB110-60986]

Western Blot: Tie-2 Antibody (MM0018-21G7) - Azide and BSA Free [NB110-60986] - Effects of proteasome inhibitor ALLN (dose-dependent) on the vascular receptor protein expression in HUVECs.
Immunohistochemistry: Tie-2 Antibody (MM0018-21G7) - Azide and BSA Free [NB110-60986]

Immunohistochemistry: Tie-2 Antibody (MM0018-21G7) - Azide and BSA Free [NB110-60986]

Immunohistochemistry: Tie-2 Antibody (MM0018-21G7) - Azide and BSA Free [NB110-60986] - Formalin fixed and paraffin embedded human placental tissue section is subjected to IHC with anti human Tie-2.
Immunohistochemistry-Paraffin: Tie-2 Antibody (MM0018-21G7) - Azide and BSA Free [NB110-60986]

Immunohistochemistry-Paraffin: Tie-2 Antibody (MM0018-21G7) - Azide and BSA Free [NB110-60986]

Immunohistochemistry-Paraffin: Tie-2 Antibody (MM0018-21G7) [NB110-60986] - Human lymph node. Tissue section pre-treated with proteinase K for 5 minutes at 37C. Antibody at 1:50. Detection with tyramide amplification system (DAKO CSA II). IHC-P image submitted by a verified customer review.
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Applications for Tie-2 Antibody (MM0018-21G7) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:200 - 1:400

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NB110-60986 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.2 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Tie-2

TIE2 (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin-like loops separated by three epidermal growth factor-like repeats that are connected to three fibronectin type III-like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2 are associated with inherited venous malformations; the TIE2 signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.

Long Name

Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2

Alternate Names

CD202b, TEK, Tie2

Entrez Gene IDs

7010 (Human)

Gene Symbol

TEK

UniProt

Q02763

Additional Tie-2 Products

Product Documents for Tie-2 Antibody (MM0018-21G7) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Tie-2 Antibody (MM0018-21G7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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