TIF1 gamma Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13036
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (97%), Mouse (97%), Xenopus (91%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human TIF1 gamma. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
123 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Centrifuge briefly prior to opening.
Scientific Data Images for TIF1 gamma Antibody
Western Blot: TIF1 gamma Antibody [NBP3-13036]
Western Blot: TIF1 gamma Antibody [NBP3-13036] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TIF1 gamma antibody (NBP3-13036) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: TIF1 gamma Antibody [NBP3-13036]
Immunocytochemistry/Immunofluorescence: TIF1 gamma Antibody [NBP3-13036] - TIF1 gamma antibody detects TIF1 gamma protein at nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TIF1 gamma stained by TIF1 gamma antibody (NBP3-13036) diluted at 1:2000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:500.Immunohistochemistry-Paraffin: TIF1 gamma Antibody [NBP3-13036]
Immunohistochemistry-Paraffin: TIF1 gamma Antibody [NBP3-13036] - Human breast carcinoma. TIF1 gamma stained by TIF1 gamma antibody (NBP3-13036) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.Applications for TIF1 gamma Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:3000
Application Notes
IHC-IHC-P- Assay dependent
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TIF1 gamma
Alternate Names
E3 ubiquitin-protein ligase TRIM33, EC 6.3.2, EC 6.3.2.-, Ectodermin homolog, KIAA1113, Protein Rfg7, PTC7FLJ32925, RET-fused gene 7 protein, RFG7FLJ11429, TF1G, TIF1GAMMA, TIF1-gamma, TIF1GECTO, TIFGAMMA, Transcription intermediary factor 1-gamma, transcriptional intermediary factor 1 gamma, tripartite motif containing 33, tripartite motif-containing 33, Tripartite motif-containing protein 33
Gene Symbol
TRIM33
Additional TIF1 gamma Products
Product Specific Notices for TIF1 gamma Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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