TIF1 gamma Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13036

Novus Biologicals, part of Bio-Techne
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NBP3-13036
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (97%), Mouse (97%), Xenopus (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TIF1 gamma Antibodies »

Product Summary for TIF1 gamma Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human TIF1 gamma. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

123 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for TIF1 gamma Antibody

Western Blot: TIF1 gamma Antibody [NBP3-13036]

Western Blot: TIF1 gamma Antibody [NBP3-13036]

Western Blot: TIF1 gamma Antibody [NBP3-13036] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TIF1 gamma antibody (NBP3-13036) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: TIF1 gamma Antibody [NBP3-13036]

Immunocytochemistry/ Immunofluorescence: TIF1 gamma Antibody [NBP3-13036]

Immunocytochemistry/Immunofluorescence: TIF1 gamma Antibody [NBP3-13036] - TIF1 gamma antibody detects TIF1 gamma protein at nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TIF1 gamma stained by TIF1 gamma antibody (NBP3-13036) diluted at 1:2000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:500.
Immunohistochemistry-Paraffin: TIF1 gamma Antibody [NBP3-13036]

Immunohistochemistry-Paraffin: TIF1 gamma Antibody [NBP3-13036]

Immunohistochemistry-Paraffin: TIF1 gamma Antibody [NBP3-13036] - Human breast carcinoma. TIF1 gamma stained by TIF1 gamma antibody (NBP3-13036) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Applications for TIF1 gamma Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000
Application Notes
IHC-IHC-P- Assay dependent
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TIF1 gamma

TIF1 gamma is encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described, however, the full-length nature of one variant has not been determined.

Alternate Names

E3 ubiquitin-protein ligase TRIM33, EC 6.3.2, EC 6.3.2.-, Ectodermin homolog, KIAA1113, Protein Rfg7, PTC7FLJ32925, RET-fused gene 7 protein, RFG7FLJ11429, TF1G, TIF1GAMMA, TIF1-gamma, TIF1GECTO, TIFGAMMA, Transcription intermediary factor 1-gamma, transcriptional intermediary factor 1 gamma, tripartite motif containing 33, tripartite motif-containing 33, Tripartite motif-containing protein 33

Gene Symbol

TRIM33

Additional TIF1 gamma Products

Product Documents for TIF1 gamma Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TIF1 gamma Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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