Tight Junction Protein 1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11284
Key Product Details
Species Reactivity
Human, Mouse, Canine
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit
Concentration
0.1 mg/ml
Product Specifications
Immunogen
Recombinant human GST-Tight Junction Protein 1 (aa 1-888)
Specificity
The polyclonal antibody recognizes both ZO-1 alpha-minus and alpha-plus.
Clonality
Polyclonal
Host
Rabbit
Applications for Tight Junction Protein 1 Antibody
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS, 0.1% BSA
Preservative
0.02% Sodium Azide
Concentration
0.1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Tight Junction Protein 1
ZO-1 is a peripheral membrane protein bound on the cytoplasmic surface of junctional contacts and is expressed in all tight junctions regardless of their properties. ZO-1 immunoprecipitates with its family member ZO-2. ZO-1 was shown to undergo tyrosine phosphorylation during tight junction formation and remodeling. Two different isoforms of ZO-1, alpha-minus and alpha-plus, have been described, which result from alternative splicing of an mRNA encoded by a single gene. The ZO-1 alpha-plus contains an 80 amino acids motif called alpha which is not present in ZO-1 alpha-minus. The alpha-containing isoform is found in most epithelial cell junctions. The short isoform (ZO-1 alpha-minus) is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules. This difference in distribution provides molecular distinction among tight junctions. The polyclonal antibody was raised against amino acids 432-1150 of human ZO-1 and recognizes both ZO-1 alpha-minus and alpha-plus. It cross reacts with mouse and dog ZO-1.
Alternate Names
DKFZp686M05161, MGC133289, Tight junction protein 1, tight junction protein 1 (zona occludens 1), tight junction protein ZO-1, TJP1, ZO1, ZO-1, zona occludens 1, Zona occludens protein 1, zonula occludens 1 protein, Zonula occludens protein 1
Gene Symbol
TJP1
Additional Tight Junction Protein 1 Products
Product Documents for Tight Junction Protein 1 Antibody
Product Specific Notices for Tight Junction Protein 1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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