Timeless Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32559
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: FVELLFWKNTAVVREMTEGYGSLDDRSSSRRAPTWSPEEEAHLQELYLANKDVEGQDVVEAILAHLNTVPRTRKQIIHHLVQMGLADSVKDFQR
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (84%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Timeless Antibody
![Immunocytochemistry/ Immunofluorescence: Timeless Antibody [NBP2-32559]](https://resources.bio-techne.com/images/products/Timeless-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-32559-img0008.jpg "Immunocytochemistry/ Immunofluorescence: Timeless Antibody [NBP2-32559]")
Immunocytochemistry/ Immunofluorescence: Timeless Antibody [NBP2-32559]
Immunocytochemistry/Immunofluorescence: Timeless Antibody [NBP2-32559] - Staining of human cell line MCF7 shows localization to nucleus.
Western Blot: Timeless Antibody [NBP2-32559] -
Western Blot: Timeless Antibody [NBP2-32559] -Analysis in human cell line Daudi.
Applications for Timeless Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Timeless
Alternate Names
FLJ12640, hTIMTIMELESS1, TIM1protein timeless homolog, timeless (Drosophila) homolog, timeless homolog (Drosophila), TIMFLJ20714
Gene Symbol
TIMELESS
Additional Timeless Products
Product Documents for Timeless Antibody
Product Specific Notices for Timeless Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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