TIMM50 Antibody (0W6J4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15459

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15459-100ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 0W6J4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TIMM50 Antibodies »

Product Summary for TIMM50 Antibody (0W6J4)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TIMM50 (Q3ZCQ8). MAASAAVFSRLRSGLRLGSRGLCTRLATPPRRAPDQAAEIGSRGSTKAQGPQQQPGSEGPSYAKKVALWLAGLLGAGGTVSVVYIFGNNPVDENGAKIPD

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TIMM50 Antibody (0W6J4)

Western Blot: TIMM50 Antibody (0W6J4) [NBP3-15459]

Western Blot: TIMM50 Antibody (0W6J4) [NBP3-15459]

Western Blot: TIMM50 Antibody (0W6J4) [NBP3-15459] - Western blot analysis of extracts of various cell lines, using TIMM50 Rabbit mAb (NBP3-15459) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: TIMM50 Antibody (0W6J4) [NBP3-15459]

Immunocytochemistry/ Immunofluorescence: TIMM50 Antibody (0W6J4) [NBP3-15459]

Immunocytochemistry/Immunofluorescence: TIMM50 Antibody (0W6J4) [NBP3-15459] - Immunofluorescence analysis of NIH-3T3 cells using TIMM50 Rabbit mAb (NBP3-15459) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: TIMM50 Antibody (0W6J4) [NBP3-15459]

Immunohistochemistry-Paraffin: TIMM50 Antibody (0W6J4) [NBP3-15459]

Immunohistochemistry-Paraffin: TIMM50 Antibody (0W6J4) [NBP3-15459] - Immunohistochemistry of paraffin-embedded mouse pancreas using TIMM50 Rabbit mAb (NBP3-15459) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for TIMM50 Antibody (0W6J4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TIMM50

Tim50 is a component of the yeast Tim23 import machinery, which mediates translocation of presequence containing proteins across the mitochondrial inner membrane. By searching sequence databases, open reading frames encoding proteins with similarity to Tim50 and with a putative mitochondrial presequence were identified in the genomes of evolutionarily distant organisms, including human. Tim50 was found to interact with the N terminal intermembrane space domain of Tim23. Functional defects of Tim50 either by depletion of the protein or addition of anti Tim50 antibodies blocked the protein translocation across the inner membrane. A translocation intermediate accumulated at the translocator of the outer mitochondrial membrane (TOM) complex was cross linked to Tim50. Thus it can be concluded that Tim50, in cooperation with Tim23, facilitates transfer of the translocating protein from the TOM complex to the Tim23 complex.

Alternate Names

homolog of yeast Tim50, MGC102733, mitochondrial import inner membrane translocase subunit TIM50, TIM50, TIM50L, Tim50-like protein, translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae), translocase of inner mitochondrial membrane 50 homolog (yeast)

Gene Symbol

TIMM50

Additional TIMM50 Products

Product Documents for TIMM50 Antibody (0W6J4)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TIMM50 Antibody (0W6J4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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