TIP120A Antibody (5D7)

Novus Biologicals, part of Bio-Techne | Catalog # H00055832-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5D7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TIP120A Antibodies »

Product Specifications

Immunogen

CAND1 (NP_060918, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASASYHISNLLEKMTSSDKDFRFMATNDLMTELQKDSIKLDDDSERKVVKMILKLLEDKNGEVQNLAVKCLGPLVSKVKEYQVETIVDTLCTNMLSDKE

Specificity

CAND1 - cullin-associated and neddylation-dissociated 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for TIP120A Antibody (5D7)

Western Blot: TIP120A Antibody (5D7) [H00055832-M01]

Western Blot: TIP120A Antibody (5D7) [H00055832-M01]

Western Blot: TIP120A Antibody (5D7) [H00055832-M01] - CAND1 monoclonal antibody (M01), clone 5D7 Analysis of CAND1 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: TIP120A Antibody (5D7) [H00055832-M01]

Immunocytochemistry/ Immunofluorescence: TIP120A Antibody (5D7) [H00055832-M01]

Immunocytochemistry/Immunofluorescence: TIP120A Antibody (5D7) [H00055832-M01] - Analysis of monoclonal antibody to CAND1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: TIP120A Antibody (5D7) [H00055832-M01]

Immunohistochemistry-Paraffin: TIP120A Antibody (5D7) [H00055832-M01]

Immunohistochemistry-Paraffin: TIP120A Antibody (5D7) [H00055832-M01] - Analysis of monoclonal antibody to CAND1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.
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Applications for TIP120A Antibody (5D7)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TIP120A

Enhances transcription from various types of promoters . Regulatory protein that interferes with the assembly of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex and thereby down-regulates ubiquitination of target proteins. Prevents neddylation of CUL1 by physically blocking access to the neddylation site. Disrupts interactions between CUL1 and SKP1A and between CUL1 and F-box proteins

Alternate Names

cullin-associated and neddylation-dissociated 1, Cullin-associated and neddylation-dissociated protein 1, cullin-associated NEDD8-dissociated protein 1, DKFZp434M1414, FLJ90441, KIAA0829FLJ10114, p120 CAND1, TBP interacting protein, TBP-interacting protein 120A, TBP-interacting protein of 120 kDa A, TIP120AFLJ38691, TIP120FLJ10929

Entrez Gene IDs

55832 (Human)

Gene Symbol

CAND1

OMIM

607727 (Human)

UniProt

NP_060918 (Human)

Additional TIP120A Products

Product Documents for TIP120A Antibody (5D7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TIP120A Antibody (5D7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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