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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2E6

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human TIPIN (NP_060328) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

34.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TIPIN Antibody (OTI2E6) - Azide and BSA Free

Western Blot: TIPIN Antibody (OTI2E6)Azide and BSA Free [NBP2-74528]

Western Blot: TIPIN Antibody (OTI2E6)Azide and BSA Free [NBP2-74528]

Western Blot: TIPIN Antibody (OTI2E6) - Azide and BSA Free [NBP2-74528] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TIPIN.
Immunohistochemistry: TIPIN Antibody (OTI2E6) - Azide and BSA Free [NBP2-74528]

Immunohistochemistry: TIPIN Antibody (OTI2E6) - Azide and BSA Free [NBP2-74528]

Immunohistochemistry: TIPIN Antibody (OTI2E6) - Azide and BSA Free [NBP2-74528] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: TIPIN Antibody (OTI2E6) - Azide and BSA Free [NBP2-74528]

Immunohistochemistry: TIPIN Antibody (OTI2E6) - Azide and BSA Free [NBP2-74528]

Immunohistochemistry: TIPIN Antibody (OTI2E6) - Azide and BSA Free [NBP2-74528] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Applications for TIPIN Antibody (OTI2E6) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TIPIN

Timeless-interacting protein (TIPIN) was originally isolated as a protein that interacts with timeless, a factor that plays a role in circadian rhythm and cell cycle checkpoint regulation. The TIM-TIPIN complex has been shown to coordinate the intra-S-phase checkpoint in response to UV-induced DNA damage. TIPIN is required for cell cycle arrest in response to DNA damage. It also interacts with RPA (replication protein A) on DNA and is able to slow down DNA chain elongation in response to UV-damage.

Alternate Names

FLJ20516, TIMELESS interacting protein, TIMELESS-interacting protein

Gene Symbol

TIPIN

Additional TIPIN Products

Product Documents for TIPIN Antibody (OTI2E6) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TIPIN Antibody (OTI2E6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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