TLE1 Antibody (TLE1/2085)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07727

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # TLE1/2085

Concentration

0.2 mg/ml

View all TLE1 Antibodies »

Product Specifications

Immunogen

Recombinant fragment (aa 175-338) of human TLE1 (exact sequence is proprietary) (Uniprot: Q04724)

Localization

Nuclear

Marker

Synovial Sarcoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08727)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for TLE1 Antibody (TLE1/2085)

Western Blot: TLE1 Antibody (TLE1/2085) [NBP3-07727]

Western Blot: TLE1 Antibody (TLE1/2085) [NBP3-07727]

Western Blot: TLE1 Antibody (TLE1/2085) [NBP3-07727] - Western Blot Analysis of human HeLa cell lysate using TLE1 Mouse Monoclonal Antibody (TLE1/2085).
Immunohistochemistry-Paraffin: TLE1 Antibody (TLE1/2085) [NBP3-07727]

Immunohistochemistry-Paraffin: TLE1 Antibody (TLE1/2085) [NBP3-07727]

Immunohistochemistry-Paraffin: TLE1 Antibody (TLE1/2085) [NBP3-07727] - Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with TLE1 Mouse Monoclonal Antibody (TLE1/2085).
Protein Array: TLE1 Antibody (TLE1/2085) [NBP3-07727]

Protein Array: TLE1 Antibody (TLE1/2085) [NBP3-07727]

Protein Array: TLE1 Antibody (TLE1/2085) [NBP3-07727] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TLE1 Mouse Monoclonal Antibody (TLE1/2085).

Applications for TLE1 Antibody (TLE1/2085)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: TLE1

Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG

Long Name

Transducin-like Enhancer of Split 1

Alternate Names

GRG1

Gene Symbol

TLE1

UniProt

Q04724 (Human)

Additional TLE1 Products

Product Documents for TLE1 Antibody (TLE1/2085)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TLE1 Antibody (TLE1/2085)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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