Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all TLE1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 175 and 225 of human Transducin-like Enhancer of Split 1 using the numbering given in entry NP_005068.2 (GeneID 7088).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TLE1 Antibody

Immunohistochemistry-Paraffin: TLE1 Antibody [NBP2-04105]

Immunohistochemistry-Paraffin: TLE1 Antibody [NBP2-04105]

Immunohistochemistry-Paraffin: TLE1 Antibody [NBP2-04105] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- TLE1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
Immunoprecipitation: TLE1 Antibody [NBP2-04105]

Immunoprecipitation: TLE1 Antibody [NBP2-04105]

Immunoprecipitation: TLE1 Antibody [NBP2-04105] - Samples: Whole cell lysate from 293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TLE1 antibody NBP2-04105 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. TLE1 was also immunoprecipitated by rabbit anti-TLE1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Applications for TLE1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TLE1

Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG

Long Name

Transducin-like Enhancer of Split 1

Alternate Names

GRG1

Gene Symbol

TLE1

UniProt

NP_005068.2 (Human)

Additional TLE1 Products

Product Documents for TLE1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TLE1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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